Listerien-Selektivagar Palcam
Nach § 64 LFGB
| Shorthand symbol: | LSP |
| Item number: | 40-1209 |
| Format: | Petri dish, 90mm |
| Colour: | Red, transparent |
| Storage conditions: | Dry, Closed at 4-10°C |
| Shelf-life: | 2 Months |
| pH: | 7.2 ± 0.2 at 25°C |
The Listeria Selektivagar Palcam is used for the isolation and detection of Listeria monocytogenes from food (§ 64 LFGB), biological sample material and heavily contaminated samples from the environment.
| in g per 1l Medium | |
|---|---|
| Peptone | 23 |
| Starch | 1 |
| Sodium chloride | 5 |
| Yeast extract | 3 |
| D-Glucose | 0.5 |
| D-Mannitol | 10 |
| Esculin | 0.8 |
| Ammonium Iron(III)-citrate | 0,5 |
| Lithium chloride | 15 |
| Phenol red | 0.08 |
| Acriflavin-Hydrochloride | 0.005 |
| Agar | 12 |
| *Adjusted as required to meet performance standards |
Themicrobial performance test is carried out in conformity with the requirementsof DIN EN ISO 11133.
Productivity
Inkubation conditions: 44 ± 4 hours at 37 ± 1 °C; Inoculation concentration: 80 – 120 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Listeria monocytogenes 4b | ATCC 13932 / WDCM 00021 | 50 – 130 % | Grey green to black colony with black background |
Selectivity
Inkubationsbedingungen: 44 ± 4 Stunden bei 37 ± 1 °C; Beimpfungskonzentration: 10.000 – 1.000.000 KBE
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739 / WDCM 00012 | Complete inhibition | - | |
| Enterococcus faecalis | ATCC 19433 / WDCM 00009 | Complete inhibition | - |
Specificity
Incubation conditions: 44 ± 4 hours at 37 ± 1 °C; Inoculations concentration: 1.000 – 10.000 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Listeria innocua | ATCC 33090 / WDCM 00017 | 50 – 130 % | Grey green to black colonies with black background |
Microbial Contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 at 30 – 35 °C
| Specification |
|---|
| Without microbial contamination |