Tryptose-Sulfit-Cycloserin-Agar (TSC)


Shorthand symbol: TSC
Item number: 40-1241
Format: Petri Dish, 90mm
Colour: Light beige
Storage conditions: Dry, in closed bag, at 4 – 10°C.
Shelf-life: 4 Weeks
pH: 7.6 ± 0.2 at 25°C
Tsc
Intented use and fields of application

Tryptose-Sulfit-Cycloserin-Agar is used for the presumptive identification and enumeration of Clostridium perfringens and other sulphite-reducing Clostridia in food, water and clinical samples. This growth medium contains nutrients to promote the growth of Clostridia and ensure their rapid proliferation. The inclusion of Ammonium-Ferric-(III)-Citrate and the Sodium Metabisulphite are indicators of sulphite reduction by Clostridium perfringens which produces black colonies.


Typical composition in g/l

in g per 1 litre of nutrient medium
Tryptose15.0
Soya Bean Peptone5.0
Yeast Extract5.0
Sodium Metabisulphite, anhydrous1.0
Ammonium Ferric (III) Citrate1.0
D-Cycloserine0.4
Agar12.0


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements ISO 11133.


Productivity
Incubation conditions: 21 ± 3 Hours at 44 ± 1 °C; Inoculation concentration 80–120 CFU; Anaerobic incubation.

OrganismType StrainSpecificationColony morphology
Clostridium perfringensATCC 13124 / WDCM 0000750 – 130 %Black colonies


Selectivity
Incubation conditions: 21 ± 3 Hours at 44 ± 1 °C; Inoculation concentration: 10,000 – 1,000,000 CFU; Anaerobic incubation

OrganismType StrainSpecificationColony morphology
Bacillus subtilisATCC 6633 / WDCM 00003Complete inhibition. -


Microbial Contamination
Incubation conditions: 2–7 days, 20–25 °C and 5–7 days, 30–35 °C

Specification
No microbial contamination


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