Tryptose-Sulfit-Cycloserin-Agar (TSC-Agar) (TSC)
| Shorthand symbol: | TSC |
| Item number: | 40-1241 |
| Format: | Petri Dish, 90mm |
| Colour: | Light beige |
| Storage conditions: | Dry, in closed bag, at 4 – 10°C. |
| Shelf-life: | 4 Weeks |
| pH: | 7.6 ± 0.2 at 25°C |
Tryptose-Sulfit-Cycloserin-Agar is used for the presumptive identification and enumeration of Clostridium perfringens and other sulfite-reducing Clostridia in food, water and clinical samples. It contains nutrients to promote the growth of Clostridia and ensure their rapid proliferation. The inclusion of Ammonium-Iron-(III)-Citrate and the Sodium Meta bisulphite are indicators of sulphite reduction by Clostridium perfringens which produces black colonies.
| in g per 1 litre of nutrient medium | |
|---|---|
| Tryptose | 15.0 |
| Soya Bean Peptone | 5.0 |
| Yeast Extract | 5.0 |
| Sodium Meta bisulphite, anhydrous | 1.0 |
| Ammonium Iron (III) Citrate | 1.0 |
| D-Cycloserine | 0.4 |
| Agar | 12.0 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance Test is carried out in accordance with the requirements ISO 11133.
Productivity
Incubation conditions: 21 ± 3 hours at 44 ± 1 °C; Inoculation concentration 80–120 CFU; Anaerobic incubation.
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Clostridium perfringens | ATCC 13124 / WDCM 00007 | 50 – 130 % | Black colonies |
Selectivity
Incubation conditions: 21 ± 3 Hours at 44 ± 1 °C; Inoculum concentration: 10,000 – 1,000,000 CFU; Anaerobic incubation
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Bacillus subtilis | ATCC 6633 / WDCM 00003 | Complete inhibition. | - |
Microbial Contamination
Incubation conditions: 2–7 days at 20–25 °C and 5–7 days at 30–35 °C
| Specification |
|---|
| No microbial contamination |