Tryptose-Sulfit-Cycloserin-Agar (TSC)

Shorthand symbol: TSC
Item number: 40-1241
Format: Petri Dish, 90mm
Colour: Light beige
Storage conditions: Dry, in closed bag, at 4 – 10°C.
Shelf-life: 4 Weeks
pH: 7.6 ± 0.2 at 25°C
Intented use and fields of application

Tryptose-Sulfit-Cycloserin-Agar is used for the presumptive identification and enumeration of Clostridium perfringens and other sulphite-reducing Clostridia in food, water and clinical samples. This growth medium contains nutrients to promote the growth of Clostridia and ensure their rapid proliferation. The inclusion of Ammonium-Ferric-(III)-Citrate and the Sodium Metabisulphite are indicators of sulphite reduction by Clostridium perfringens which produces black colonies.

Typical composition in g/l

in g per 1 litre of nutrient medium
Soya Bean Peptone5.0
Yeast Extract5.0
Sodium Metabisulphite, anhydrous1.0
Ammonium Ferric (III) Citrate1.0

Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements ISO 11133.

Incubation conditions: 21 ± 3 Hours at 44 ± 1 °C; Inoculation concentration 80–120 CFU; Anaerobic incubation.

OrganismType StrainSpecificationColony morphology
Clostridium perfringensATCC 13124 / WDCM 0000750 – 130 %Black colonies

Incubation conditions: 21 ± 3 Hours at 44 ± 1 °C; Inoculation concentration: 10,000 – 1,000,000 CFU; Anaerobic incubation

OrganismType StrainSpecificationColony morphology
Bacillus subtilisATCC 6633 / WDCM 00003Complete inhibition. -

Microbial Contamination
Incubation conditions: 2–7 days, 20–25 °C and 5–7 days, 30–35 °C

No microbial contamination

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