Trypton-Glucose-Hefeextrakt-Agar, Plate-Count-Agar (TGE)


Shorthand symbol: TGE
Item number: 40-1189
Format: Petri Dish, 90mm
Colour: Yellowish
Storage conditions: Dry, in closed bag, at 4-10°C
Shelf-life: 4 Months
pH: 7.0 ± 0.2 at 25°C
Tge
Intented use and fields of application

Largely the inhibitor-free nutrient medium Tryptone Glucose Yeast Extract Agar enables the growth of bacteria and yeasts. Inoculation of the culture medium is carried out either with the original material or the spatula with diluents.


Typical composition in g/l

in g per 1 litre of Nutrient medium
Caseine Peptone5
Yeast Extract2.5
Glucose1
Agar10
*Adjusted as required to meet performance standards


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of EN ISO 11133:2014.


Productivity
Incubation conditions: 72±3 hours at 30±1°C; Inoculum concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Bacillus subtilisATCC 6633/WDCM 00003>70%Medium growth, whitish colonies
Escherichia coliATCC 8739/WDCM 00012>70%Medium growth, whitish colonies
Staphylococcus aureusATCC 25923/WDCM 00034>70%Light, whitish-yellow colonies


Microbial Contamination
Incubation conditions: 5–7 days, 20–25°C and 5–7 days, 30–35°C

Specification
No microbial contamination


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