Tetrathionat-Bouillon nach Muller-Kauffmann (MKTT)

Shorthand symbol: MKTT
Item number: 60-1187
Format: Tube, 10ml
Colour: green, opaque
Storage conditions: Dry, in closed bag, at 15-22°C
Shelf-life: 3 Months
pH: 7.6 ± 0.2 at 25°C
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Intented use and fields of application

Tetra Thionate Broth of Muller-Kauffmann is for the selective isolation and cultivation of Salmonella in meat, meat products, and other foodstuffs.

Typical composition in g/l

in g per 1 Litre Nutrient Medium
Casein Peptone10
Meat Extract5
Ox bile5
Calcium Carbonate45
Sodium Thiosulphate31.85
Sodium Chloride3
Brillant Green0.01
Potassium Iodide5
*Adjusted as required to meet performance standards

Microbiological quality control

The Microbiological Performance test is carried out in accordance with the requirements of DIN EN ISO 11133.


Incubation Conditions: 24±3 Hours, 37±1°C; Inoculum Concentration: 100 CFU

OrganismType StrainSpecificationColony morphology
Salmonella enterica serotype Enteritidis
+ Escherichia coli
+ Pseudomonas aeroginosa
ATCC 13076/WDCM 00030
+ ATCC 8739/WDCM 00012
+ ATCC 27853/WDCM 00025
> 10 Colonies Kolonien on XLD-AgarColonies with black centre and a slightly transparent reddish zone due to the change of the medium (XLD)

Incubation Conditions: 24±3 hours at 37±1°C; Inoculum Concentration: 10.000-1.000.000 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739/WDCM 00012= 100 Colonies on TSA-
Enterococcus faecalisATCC 19433/WDCM 00009< 10 Colonies on TSA-

Microbial Contamination

Incubation Conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C

No microbial contamination

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