Tetrathionat-Bouillon nach Muller-Kauffmann (MKTT)
| Shorthand symbol: | MKTT |
| Item number: | 60-1187 |
| Format: | Tube, 10ml |
| Colour: | green, opaque |
| Storage conditions: | Dry, in closed bag, at 15-22°C |
| Shelf-life: | 3 Months |
| pH: | 7.6 ± 0.2 at 25°C |
Tetra Thionate Broth of Muller-Kauffmann is for the selective isolation and cultivation of Salmonella in meat, meat products, and other foodstuffs.
| in g per 1 Litre Nutrient Medium | |
|---|---|
| Casein Peptone | 10 |
| Meat Extract | 5 |
| Ox bile | 5 |
| Calcium Carbonate | 45 |
| Sodium Thiosulphate | 31.85 |
| Sodium Chloride | 3 |
| Brillant Green | 0.01 |
| Iodine | 4 |
| Potassium Iodide | 5 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance test is carried out in accordance with the requirements of DIN EN ISO 11133.
Productivity
Incubation Conditions: 24±3 Hours, 37±1°C; Inoculum Concentration: 100 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Salmonella enterica serotype Enteritidis + Escherichia coli + Pseudomonas aeroginosa | ATCC 13076/WDCM 00030 + ATCC 8739/WDCM 00012 + ATCC 27853/WDCM 00025 | > 10 Colonies Kolonien on XLD-Agar | Colonies with black centre and a slightly transparent reddish zone due to the change of the medium (XLD) |
Selectivity
Incubation Conditions: 24±3 hours at 37±1°C; Inoculum Concentration: 10.000-1.000.000 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739/WDCM 00012 | = 100 Colonies on TSA | - | |
| Enterococcus faecalis | ATCC 19433/WDCM 00009 | < 10 Colonies on TSA | - |
Microbial Contamination
Incubation Conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C
| Specification |
|---|
| No microbial contamination |