Tetra Thionate Broth according to Muller-Kauffmann (MKTT)

Shorthand symbol: MKTT
Item number: 60-1187
Format: Tube, 10ml
Colour: green, opaque
Storage conditions: Dry, in closed bag, at 15-22°C
Shelf-life: 3 Months
pH: 7.6 ± 0.2 bei 25°C
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Intented use and fields of application

Tetra Thionate Broth of Muller-Kauffmann is for the selective isolation and cultivation of Salmonella in meat, meat products, and other foodstuffs.

Typical composition in g/l

in g per 1 Litre Nutrient Medium
Casein Peptone10
Meat Extract5
Ox bile5
Calcium Carbonate45
Sodium Thiosulphate31.85
Sodium Chloride3
Brillant Green0.01
Potassium Iodide5

Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of DIN EN ISO 11133.


Incubation Conditions: 24±3 Hours, 37±1°C; Inoculum Concentration: 100 CFU

OrganismType StrainSpecificationColony morphology
Salmonella enterica serotype Enteritidis
+ Escherichia coli
+ Pseudomonas aeroginosa
ATCC 13076/WDCM 00030
+ ATCC 8739/WDCM 00012
+ ATCC 27853/WDCM 00025
> 10 Colonies Kolonien on XLD-AgarColonies with black centre and a slightly transparent reddish zone due to the change of the medium (XLD)

Incubation Conditions: 24±3 Hours, 37±1°C; Inoculum Concentration: 10,000-1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739/WDCM 00012= 100 Colonies on TSA-
Enterococcus faecalisATCC 19433/WDCM 00009< 10 Colonies on TSA-

Microbial Contamination

Incubation Conditions: 5–7 Days, 20–25°C and 5–7 Days, 30–35°C

No microbial contamination

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