Streptococcus-Selective-Agar (SESA)


Shorthand symbol: SESA
Item number: 40-1507
Format: Petri Dish, 90mm
Colour: Blood-red, opaque
Storage conditions: Dry, in closed bag, at 4 – 10°C.
Shelf-life: 2 Months
pH: 7.3 ± 0.2 at 25°C
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Intented use and fields of application

Streptococcus Selective Agar allows the isolation of Streptococci species and Enterococci species from clinical materials and foods.


Typical composition in g/l

in g per 1 Litre of Nutrient medium
Casein peptone15.0
Soya peptone5.0
Sodium chloride5.0
Aesculin1.0
Ammonium Ferric (III) citrate0.5
Colistine0.01
Oxalic acid0.005
Neomycin sulphate0.02
Sheep's blood (in ml)50.0
Agar17.0


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the following specification:


Produktivität

Incubation Conditions: 24 ± 4 Hours at 36 ± 2 °C; Inoculum Concentration of Target Organisms: 80 – 120 CFU

OrganismType StrainSpecificationColony morphology
Streptococcus pyogenesATCC 1234450–130 %Small, grey colonies with significant hydrolysis
Enterococcus faecalisATCC 19433/WDCM 0000950–130 %Small, grey colonies with blackening of the agar media around the colonies


Selectivity

Incubation conditions: 24±4 hours at 36±2°C; Inoculum concentration: Target organisms: 10.000–1.000.000 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739/WDCM 00012Complete or partial inhibition-
Staphylococcus aureusATCC 6538/WDCM 00032Complete or partial inhibition-


Microbial Contamination
Incubation conditions: 5–7 days, 20–25°C and 5–7 days, 30–35 °C

Specification
No microbial contamination


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