Salmonella-Shigella-Agar, modifiziert (SS-Agar)
| Shorthand symbol: | SSA |
| Item number: | 40-1221 |
| Format: | Petri dish, 90mm |
| Colour: | Red brown, transparent |
| Storage conditions: | Dry, closed at 4-10°C |
| Shelf-life: | 4 Weeks |
| pH: | 7.3 ± 0.2 at 25°C |
Medium for the isolation of Salmonella and Shigella. The growth of gram-positive microorganisms is inhibited and the swarming of Proteus ssp. is suppressed.
| in g per 1l Medium | |
|---|---|
| Peptone | 7.7 |
| Meat extract | 5 |
| Yeast extract | 2.3 |
| Beef Bile | 8.5 |
| Lactose | 10 |
| Sodium citrate | 5 |
| Sodium thiosulphate | 7 |
| Ammonium Iron(III)-citrate | 1 |
| Brillant green | 0.003 |
| Neutral red | 0.025 |
| Agar | 10 |
| *Adjusted as required to meet performance standards |
Themicrobial performance test is carried out in conformity with the requirementsof DIN EN ISO 11133.
Productivity
Incubation conditions: 24 ± 3 hours at 37 ± 1 °C; Inoculation concentration: 80 – 120 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Salmonella enterica serovar Enteritidis | ATCC 13076 / WDCM 00030 | 50 – 130 % | Colorless colonies with black center | |
| Shigella flexneri | ATCC 29903 / WDCM 00125 | 50 – 130 % | Small, colorless colonies - unchanged medium |
Selektivität
Incubation conditions: 24 ± 3 hours at 37 ± 1 °C; Inoculation concentration: 10.000 – 1.000.000 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739 / WDCM 00012 | Inhibited | Red, opaque colonies with partially red precipitate | |
| Staphylococcus aureus | ATCC 6538 / WDCM 00032 | Complete inhibited | - |
Microbial Contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| Specification |
|---|
| Without microbial contamination |