Sabouraud-Dextrose-Agar nach harm. EP/USP/JP (SAB)
|Format:||Petri Dish, 90mm|
|Storage conditions:||Dry, in closed bag at 15 - 22°C|
|pH:||5.6 ± 0.2 at 25°C|
Universal Nutrient medium for the cultivation and enumeration of moulds and yeasts and dematophytes. Sabouraud Dextrose Agar contains 4% dextrose, which promotes the growth of these microorganisms. The low pH promotes spore and pigment formation of yeast and fungi, and inhibits the growth of bacteria. It therefore is a selective culture.
This Nutrient medium complies with the requirements of the methods of the EP/USP/JP. The use of Sabouraud Dextrose Agar medium according to the EP/USP/JP is recommended for the full analysis of Candida albicans in non-sterile pharmaceutical products.
|in g per 1 litre Nutrient medium|
|Pancreatic-digest of Casein Peptone||5.0|
|Pancreatic-digest of Beef Extract Peptone||5.0|
|*Adjusted as required to meet performance standards|
The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and Pharm. Eur. (Microbiological Examination of non-sterile products acording to Chapter 2.6.13).
Incubation conditions: 2–3 days at 30–35°C; Inoculum concentration: 10–100 CFU
|Organism||Type Strain||Specification||Colony morphology|
|Candida albicans||ATCC 10231/WDCM 00054||50–200 %||Whitish, dry colonies|
|Aspergillus brasiliensis||ATCC 16404/WDCM 00053||50–200 %||Brown, black conidia on mycelium|
(3–5 days, 20–25°C)
|ATCC 10231/WDCM 00054||50–200 %||Whitish, dry colonies|
(3 – 5 days, 20 – 25 °C)
|ATCC 16404/WDCM 00053||50–200 %||Brown, black conidia on mycelium|
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C
|No microbial contamination|