Sabouraud-Dextrose-Agar nach harm. EP/USP/JP
Corresponds to the recommendations of the harmonization according to EP / USP / JP
| Shorthand symbol: | SAB |
| Item number: | 50-1264 |
| Format: | Contact plate |
| Colour: | Yellowish |
| Storage conditions: | Dry, closed, at 15...22°C |
| Shelf-life: | 3 Months |
| pH: | 5.6 ± 0.2 at 25°C |
Sabouraud-Dextrose-Agar is a universal nutrient medium used for the cultivation and total count of yeasts, molds and dermatophytes. Sabouraud Dextrose Agar contains 4% dextrose, which promotes the growth of these microorganisms. The low pH promotes the sporulation and pigmentation of yeasts and molds and at the same time, it simultaneously inhibits the growth of bacteria. It is thus a Selektive nutrient medium.The nutrient medium complies with the recommendations of the harmonization according to EP / USP / JP. The use of Sabouraud dextrose agar to harm. EP / USP / JP is recommended for all tests for Candida albicans in non-sterile pharmaceutical products.
| in g pro 1l Nährmedium | |
|---|---|
| Caseinpepton, pankreatisch verdaut | 5 |
| Fleischpepton, peptisch verdaut | 5 |
| Dextrose | 40 |
| Agar | 15 |
| *Adjusted as required to meet performance standards |
The microbial performance test is carried out in conformity with the requirements of DIN EN ISO 11133 and the Pharm. Eur. (Microbiological testing of non-sterile products according to Chapter 2.6.13).
Productivity
Incubation conditions: 2 – 3 days at 30 – 35 °C; Inoculum concentration: 10 – 100 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Candida albicans | ATCC 10231 / WDCM 00054 | 50 – 200 % | Whitish, dry colonies | |
| Aspergillus brasiliensis | ATCC 16404 / WDCM 00053 | 50 – 200 % | Brown and black colonies on a bright mycelium | |
| Candida albicans (3 – 5 days, 20 – 25 °C) | ATCC 10231 / WDCM 00054 | 50 – 200 % | Whitish dry colonies | |
| Aspergillus brasiliensis (3 – 5 days, 20 – 25 °C) | ATCC 16404 / WDCM 00053 | 50 – 200 % | Brown and black colonies on a bright mycelium |
Microbial Contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| Specification |
|---|
| No microbial contamination |