Sabouraud-Dextrose-Agar acc. to EP/USP/JP with LTH (SAB-LTH)

Recommended by the Harmonised European Pharmacopoeia, supplemented with Lecithin, Polysorbate 80 (Tween 80) and L-Histidine

Shorthand symbol: SAB-LTH
Item number: 40-1532
Format: Petri Dish, 90mm
Colour: Yellowish
Storage conditions: Dry, in closed bag, at 15 – 22°C.
Shelf-life: 6 Months
pH: 5.6 ± 0.2 at 25°C
1532 sabouraud dextrose agar mit enthemmer sab lth xebios
Intented use and fields of application

Universal Nutrient medium for the isoaltion and cultivation and enumeration of yeast and moulds and dermatophyta. Sabouraud Dextrose Agar supplemented with 4% Dextrose promotes the growth of these microorganisms. The low pH promotes spore development and pigment formation in yeasts and fungi and additionally inhibits the growth of bacteria. Thus SAD is a selective growth medium.

This Nutrient medium meets the requirments for the methods of the EP/USP/JP. The use of Sabouraud Dextrose Agar is recommended for the analysis of Candida albicans in non-sterile pharmaceuticals.

The addition of Lecithin, Tween 80 and Histidine components neutralise the inhibotory effects of aldehydes, phenols, Hexachlorophene, chlorhexidine, formaldehyde and quarternary ammonium compounds used for preservatives and cleaning and disinfection agents.

Typical composition in g/l

in g per 1 Litre of Nutrient medium
Pancreatic-digest of Casein peptone5.0
Peptic-digest of Beef Peptone5.0
Tween 805.0

Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of Pharm. Eur. (Microbiological Examination of non-sterile products acording to Chapter 2.6.13).

Incubation conditions: 2–3 days at 30–35°C; Inoculum concentration: 10–100 CFU

OrganismType StrainSpecificationColony morphology
Candida albicansATCC 10231/WDCM 0005450–200 %Whitish, dry colonies
Aspergillus brasiliensisATCC 16404/WDCM 0005350–200 %Brown, black conidia on mycelium
Candida albicans
(3–5 days, 20–25°C)
ATCC 10231/WDCM 0005450–200 %Whitish, dry colonies
Aspergillus brasiliensis
(3 – 5 Tage, 20 – 25 °C)
ATCC 16404/WDCM 0005350–200 %Brown, black conidia on mycelium

Microbial Contamination
Incubation conditions: 5–7 days, 20–25°C and 5–7 days, 30–35°C

No microbial contamination

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