Sabouraud-Dextrose-Agar acc. to EP/USP/JP with LTH (SAB-LTH)
Recommended by the Harmonised European Pharmacopoeia, supplemented with Lecithin, Polysorbate 80 (Tween 80) and L-Histidine
|Format:||Petri Dish, 90mm|
|Storage conditions:||Dry, in closed bag, at 15 – 22°C.|
|pH:||5.6 ± 0.2 at 25°C|
Sabouraud-Dextrose-Agar is a universal nutrient medium used for the isoaltion and cultivation, enumeration of yeast and moulds and dermatophyta. Sabouraud Dextrose Agar is supplemented with 4% Dextrose in order to promote the growth of the microorganisms. The low pH promotes spore development and pigment formation in yeasts and fungi which in addition, inhibits the growth of bacteria. Thus SAD is a selective growth medium.
This Nutrient medium meets the requirments for the methods of the EP/USP/JP. The use of Sabouraud Dextrose Agar is recommended for the analysis of candida albicans in non-sterile pharmaceuticals.
The addition of Lecithin, Tween 80 and Histidine components neutralise the inhibitory effects of aldehydes, phenols, Hexachlorophene, chlorhexidine, formaldehyde and quarternary ammonium compounds used for preservatives, cleaning and disinfection agents.
|in g per 1 Litre of Nutrient medium|
|Pancreatic-digest of Casein peptone||5.0|
|Peptic-digest of Beef Peptone||5.0|
|*Adjusted as required to meet performance standards|
The Microbiological Performance Test is carried out in accordance with the requirements of Pharm. Eur. (Microbiological Examination of non-sterile products acording to Chapter 2.6.13).
Incubation conditions: 2–3 days at 30–35°C; Inoculum concentration: 10–100 CFU
|Organism||Type Strain||Specification||Colony morphology|
|Candida albicans||ATCC 10231/WDCM 00054||50–200 %||Whitish, dry colonies|
|Aspergillus brasiliensis||ATCC 16404/WDCM 00053||50–200 %||Brown, black conidia on mycelium|
(3–5 days, 20–25°C)
|ATCC 10231/WDCM 00054||50–200 %||Whitish, dry colonies|
(3 – 5 Tage, 20 – 25 °C)
|ATCC 16404/WDCM 00053||50–200 %||Brown, black conidia on mycelium|
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C
|No microbial contamination|