Ringerlösung (RIL)


Shorthand symbol: RIL
Item number: 60-1550
Format: Tube, 9ml
Colour: clear, colourless
Storage conditions: Dry, closed at 4-25°C
Shelf-life: 12 Months
pH: 6.9 ± 0.5 bei 25°C
Ril
Intented use and fields of application

Ringer's Solution is for the isolation and cultivation of susceptible bacteria. It's composition prevents the microorganisms suffering from osmotic shock or being damaged when they are taken out of their usual environment.


Typical composition in g/l

in g per 1 Litre of Nutrient Medium
Sodium Hydrogen Carbonate0.01
Calcium Chloride0.12
Potassium Chloride0.105
Sodium Chloride2.25


Microbiological quality control

The microbial performance test is carried out in accordance with the requirements of DIN EN ISO 11133.


Productivity
Incubation Conditions: max. 45 min - 1 hour at 25°C, then transfer on TSA, 40-48h at 37°C; Inoculum Concentration: 10CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 25922/WDCM 00013-White/grey colonies on TSA
Staphylococcus aureusATCC 25923/WDCM 00034-White/grey colonies on TSA
Microbial Contamination
Incubation conditions: 48 ± 4h at 30 ± 1°C
Specification
No microbial contamination


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