Rappaport-Vassiliadis-Salm.-Anr.bouillon (RVS) (RVS)

Shorthand symbol: RVS
Item number: 60-1262
Format: Tube, 9ml
Colour: turquoise, transparent
Storage conditions: Dry, in closed bag, at 15-22°C
Shelf-life: 2 Months
pH: 5.2 ± 0.2 bei 25°C
1262 rappaport vassiliadis salmonella anreicherungsbouillon rvs xebios
Intented use and fields of application

The RVS Broth (Rappaport-Vassiliadis Salmonella Enrichment Broth according to the harmonised EP/USP/JP) is for the selective enrichment of Salmonella. This Medium complies with the recommendations of the harmonised EP/USP/JP (2006).

Typical composition in g/l

in g per 1 Litre of Nutrient medium
Soya Peptone4.5
Magnesium Chloride, anhydrous13.58
Sodium Chloride8
Dipotassium Phosphate0.4
Potassium Dihydrogen Phosphate0.6
Malachite Green0.036
*Adjusted as required to meet performance standards

Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of DIN EN ISO 11133:2014.


Incubation Conditions: 24±3 Hours at 41.5±1°C; Inoculum Concentration: 100 CFU

OrganismType StrainSpecificationColony morphology
Salmonella enterica serotype Enteritidis
+ Escherichia coli
+ Pseudomonas aeroginosa
ATCC 13076/WDCM 00030
+ ATCC 8739/WDCM 00012
+ ATCC 27853/WDCM 00025
> 10 colonies from XLD-AgarColonies with a black centre and a lightly transparent reddish zone due to the colour change of the medium(XLD)

Incubation Conditions: 24±3 Hours at 41.5±1°C; Inoculum Concentration: 10,000 - 1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739/WDCM 00012< 100 Colonies in TSA-
Enterococcus faecalisATCC 19433/WDCM 00009< 10 Colonies in TSA-

Microbial Contamination

Incubation Conditions: 5–7 Days, 20–25°C and 5–7 Days, 30–35°C

No microbial contaminatkion

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