Pseudomonas-CN-Agar
| Shorthand symbol: | PCN |
| Item number: | 45-1286 |
| Format: | Petri Dish, 70mm |
| Colour: | colourless |
| Storage conditions: | Dry, in closed bag at 4-10°C |
| Shelf-life: | 3 Months |
| pH: | 6.8 ± 0.2 bei 25°C |
Pseudomas CN Agar is a selective Agar for the cultivation of Pseudomonas aeruginosa with the addition of Cetrimide to suppress the growth of non-target bacteria, such as Klebsiella, Proteus or other prominent species in water.
| in g per 1 litre of nutrient medium | |
|---|---|
| Gelatin Peptone | 16 |
| Casein Hydrolysate | 10 |
| Potassium Sulphate, anhydrous | 10 |
| Magnesium Chloride, anhydrous | 1.4 |
| Agar | 11 |
| Cetrimide (in mg) | 200 |
| Nalidixic Acid (in mg) | 15 |
| Glycerine (in ml) | 10 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).
Productivity
Incubation conditions: 2–3 days at 30–35 °C; Inoculum concentration: 80–120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Pseudomonas aeruginosa | ATCC 10145 / WDCM 00024 | 50 – 130 % | Blue-Green to greenish colonies with fluorescence under UV light (360 ± 20 nm) |
Selectivity
Incubation conditions: 44 ± 4 Hours at 36 ± 2 °C; Inoculum concentration: 10,000–1,000,000 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Enterococcus faecalis | ATCC 19433 / WDCM 00009 | Complete inhibition | - | |
| Escherichia coli | Complete inhibition |
Microbial Contamination
Incubation conditions: 5–7 days at 20–25 °C and 5–7 days at 30–35 °C
| Specification |
|---|
| No microbial contamination |