Pseudomonas CN Agar - 70mm-Petri Dish (PCN)


Shorthand symbol: PCN
Item number: 45-1286
Format: Petri Dish, 70mm
Colour: colourless
Storage conditions: Dry, in closed bag, at 4-10°C
Shelf-life: 3 Months
pH: 6.8 ± 0.2 bei 25°C
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Intented use and fields of application

Pseudomas CN Agar Selective Agar for the cultivation of Pseudomonas aeruginosa with the addition of Cetrimide to suppress the growth of non-target bacteria, such as Klebsiella, Proteus or other prominent species in water.


Typical composition in g/l

in g per 1 litre of nutrient medium
Gelatin Peptone16
Casein Hydrolysate10
Potassium Sulphate, anhydrous10
Magnesium Chloride, anhydrous1.4
Agar11
Cetrimide (in mg)200
Nalidixic Acid (in mg)15
Glycerine (in ml)10
*Adjusted as required to meet performance standards


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).

Productivity
Incubation conditions: 2–3 days at 30–35 °C; Inoculation concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Pseudomonas aeruginosaATCC 10145 / WDCM 0002450 – 130 %Blue-Green to greenish colonies with fluorescence under UV light (360 ± 20 nm)


Selectivity
Incubation conditions: 44 ± 4 Hours at 36 ± 2 °C; Inoculation concentration: 10,000–1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Enterococcus faecalisATCC 19433 / WDCM 00009Complete inhibition-
Escherichia coliComplete inhibition-


Microbial Contamination
Incubation conditions: 5–7 days at 20–25 °C and 5–7 days at 30–35 °C

Specification
No microbial contamination


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