Pseudomonas-CN-Agar (PCN)

According to ISO 16266


Shorthand symbol: PCN
Item number: 40-1286
Format: Petri Dish, 90mm
Colour: transparent
Storage conditions: Dry, in closed bag, at 4 – 10°C.
Shelf-life: 3 Month
pH: 6.8 ± 0.2 at 25°C
Pcn
Intented use and fields of application

Pseudomas CN Agar Selective Agar for the cultivation of Pseudomonas aeruginosa with the addition of Cetrimide to suppress the growth of non-target bacteria, such as Klebsiella, Proteus or other prominent species in water.


Typical composition in g/l

in g per 1 litre of medium
Gelatin Peptone16.0
Casein Hydrolysate10.0
Potassium Sulphate, anhydrous10.0
Magnesium Chloride, anhydrous1.4
Agar11.0
Cetrimide (in mg)200.0
Nalidixic Acid (in mg)15.0
Glycerine (in ml)10.0


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements ISO 11133:2014.


Productivity
Incubation conditions: 2–3 days at 30–35 °C; Inoculation concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Pseudomonas aeruginosaATCC 10145 / WDCM 0002450 – 130 %Blue-Green to greenish colonies with fluorescence under UV light (360 ± 20 nm)


Selectivity
Incubation conditions: 44 ± 4 Hours at 36 ± 2 °C; Inoculation concentration: 10,000–1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Enterococcus faecalisATCC 19433 / WDCM 00009Complete inhibition-
Escherichia coliComplete inhibition-


Microbial Contamination
Incubation conditions: 5–7 days at 20–25 °C and 5–7 days at 30–35 °C

Specification
No microbial contamination


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