Pseudomonas-CN-Agar (PCN)

According to ISO 16266


Shorthand symbol: PCN
Item number: 40-1286
Format: Petri Dish, 90mm
Colour: transparent
Storage conditions: Dry, in closed bag, at 4 – 10°C.
Shelf-life: 3 Month
pH: 6.8 ± 0.2 at 25°C
Pcn
Intented use and fields of application

Pseudomas CN Agar Selective Agar for the cultivation of Pseudomonas aeruginosa with the addition of Cetrimide to suppress the growth of non-target bacteria, such as Klebsiella, Proteus or other prominent species in water.


Typical composition in g/l

in g per 1 litre of Medium
Gelatin Peptone16.0
Casein Hydrolysate10.0
Potassium Sulphate, anhydrous10.0
Magnesium Chloride, anhydrous1.4
Agar11.0
Cetrimide (in mg)200.0
Nalidixic Acid (in mg)15.0
Glycerine (in ml)10.0
*Adjusted as required to meet performance standards


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements ISO 11133:2014.


Productivity
Incubation conditions: 2–3 days at 30–35 °C; Inoculation concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Pseudomonas aeruginosaATCC 10145 / WDCM 0002450 – 130 %Blue-Green to greenish colonies with fluorescence under UV light (360 ± 20 nm)


Selectivity
Incubation conditions: 44 ± 4 Hours at 36 ± 2 °C; Inoculation concentration: 10,000–1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Enterococcus faecalisATCC 19433 / WDCM 00009Complete inhibition-
Escherichia coliComplete inhibition-


Microbial Contamination
Incubation conditions: 5–7 days at 20–25 °C and 5–7 days at 30–35 °C

Specification
No microbial contamination


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