Listerien-Selektivagar Oxford
| Shorthand symbol: | LSO |
| Item number: | 40-1208 |
| Format: | Petri dish, 90mm |
| Colour: | Yellow, transparent |
| Storage conditions: | Dry, closed, at 4-10°C |
| Shelf-life: | 2 Months |
| pH: | 7.0 ± 0.2 at 25°C |
Oxford Listeria Selective Agar is used to isolate and detect Listeria monocytogenes from food and other research materials.
| in g per 1l Medium | |
|---|---|
| Peptone | 23 |
| Starch | 1 |
| Sodium chloride | 5 |
| Esculin | 1 |
| Ammonium Iron (III)-citrate | 0,5 |
| Lithium chloride | 15 |
| Acriflavine | 0.005 |
| Cycloheximide | 0.4 |
| Colistin sulphate | 0.02 |
| Cefotetan | 0.002 |
| Phosphomycin | 0.01 |
| Agar | 12 |
| *Adjusted as required to meet performance standards |
The microbial performance test is carried out inconformity with the requirements of DIN EN ISO 11133.
Productivity
Incubation conditions: 44 ± 4 Stunden bei 37 ± 1 °C; Inoculation Concentration: 80 – 120 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Listeria monocytogenes 4b | ATCC 13932 / WDCM 00021 | 50 – 130 % | Grey green to black colonies with black background |
Selectivity
Incubation conditions: 44 ± 4 hours at 37 ± 1 °C; Inoculation concentration: 10.000 – 1.000.000 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739 / WDCM 00012 | Complete Inhibition | - | |
| Enterococcus faecalis | ATCC 19433 / WDCM 00009 | Complete Inhibition | - |
Specificity
Incubation conditions: 44 ± 4 hours at 37 ± 1 °C; Inoculation concentration: 1.000 – 10.000 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Listeria innocua | ATCC 33090 / WDCM 00017 | Good growth | Grey green to black colonies with black background |
Microbial Contamination
Incubation condition: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| Specification |
|---|
| Without microbial contamination |