Natriumchlorid-Pepton-Pufferlösung (NPP)

Shorthand symbol: NPP
Item number: 60-1025
Format: Tube, 9ml
Colour: Clear, colourless
Storage conditions: Dry, in closed bag, at 15-22°C
Shelf-life: 5 Months
pH: 7.0 ± 0.2 bei 25°C
Intented use and fields of application

Dilution buffer for samples for the microbiological analysis of contaminants. Culture medium for testing non-sterile products. This medium complies with the recommendation of the harmonised method according to EP/USP/JP (2006).

Typical composition in g/l

in g per 1 Litre of Nutrient medium
Potassium Hydrogen Phosphate3.56*
Disodium Hydrogen Phosphate, anhydrous5.77*
Sodium Chloride4.3
Pancreatic-digest of Casein Peptone1
*The concentration ratio is determined by the pH setpoint.

Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of DIN EN ISO 11133.


Incubation Conditions: 2 Hours at 22.5±2.5°C; Inoculum Concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 25923/WDCM 00034--
Escherichia coliATCC 8739/WDCM 00012--

Microbial Contamination
Incubation Conditions: 5–7 Days at 20–25°C and 5–7 Days at 30–35°C

No microbial contamination

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