Natriumchlorid-Pepton-Pufferlösung (NPP)
| Shorthand symbol: | NPP |
| Item number: | 60-1025 |
| Format: | Tube, 9ml |
| Colour: | Clear, colourless |
| Storage conditions: | Dry, in closed bag, at 15-22°C |
| Shelf-life: | 5 Months |
| pH: | 7.0 ± 0.2 bei 25°C |
Dilution buffer for samples for the microbiological analysis of contaminants. Culture medium for testing non-sterile products. This medium complies with the recommendation of the harmonised method according to EP/USP/JP (2006).
| in g per 1 Litre of Nutrient medium | |
|---|---|
| Potassium Hydrogen Phosphate | 3.56* |
| Disodium Hydrogen Phosphate, anhydrous | 5.77* |
| Sodium Chloride | 4.3 |
| Pancreatic-digest of Casein Peptone | 1 |
| *The concentration ratio is determined by the pH setpoint. |
The Microbiological Performance Test is carried out in accordance with the requirements of DIN EN ISO 11133.
Dilution
Incubation Conditions: 2 Hours at 22.5±2.5°C; Inoculum Concentration: 80–120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Staphylococcus aureus | ATCC 25923/WDCM 00034 | - | - | |
| Escherichia coli | ATCC 8739/WDCM 00012 | - | - |
Microbial Contamination
Incubation Conditions: 5–7 Days at 20–25°C and 5–7 Days at 30–35°C
| Specification |
|---|
| No microbial contamination |