Nutrient Agar III (NAIII)


Shorthand symbol: NAIII
Item number: 71-1168
Format: 250ml-Bottle with 200ml nutrient medium
Colour: yellowish
Storage conditions: Dry, in closed bag, at 15 - 22°C
Shelf-life: 1 Months
pH: 7.0 ± 0.2 bei 25°C
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Intented use and fields of application

This Nutrient Agar III is for the cultivation of suspect bacteria from specimens for further biochemical and serological identification.


Typical composition in g/l

in g per 1 litre of nutrient medium
Caseine Peptone5.0
Meat Extract3.0
Agar11.0


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 1113:2014.


Productivity

Incubation conditions: 44 ± 4 Hours at 36 ± 2°C; Inoculation concentration of target organisms: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739 / WDCM 00012≥ 70 %-
Bacillus subtilisATCC 6633 / WDCM 00003≥ 70 %-


Microbial Contamination

Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C

Specification
No microbial contamination


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