Mannitol Salts Agar according to EP/USP/JP (MSA)

Shorthand symbol: MSA
Item number: 40-1166
Format: Petri Dish, 90mm
Colour: Clear and strong pink
Storage conditions: Dry, in closed bag, at 15-22°C
Shelf-life: 3 Months
pH: 7.4 ± 0.2 bei 25°C
Intented use and fields of application

Test for Staphylococcus aureus. Nutrient medium for microbiological analysis of sterile products. Medium complies with the requirments in the EP/USP/JP (2006).

Typical composition in g/l

in g per 1 litre of Nutrient medium
Pancreatic-digest of Casein peptone5
Pancreatic-digest of Beef Peptone5
Beef Extract1
Sodium chloride75
Phenol Red0.025

Microbiological quality control

The microbial performance test carried out in conformity with the requirements of Pharm. Eur. (Microbiological Examination of non-sterile products according Chapter 2.6.13).

Incubation conditions: 18 hours at 30–35°C; Inoculum concentration: 10–100 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 6538/WDCM 00032Good growthYellow colonies

Incubation conditions: 18-72 hours at 30–35°C; Inoculum concentration: > 100 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739/WDCM 00012Complete inhibition-

Microbial Contamination
Incubation conditions: 5–7 days, 20–25°C and 5–7 days, 30–35°C

No microbial contamination

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