Mannitol Salts Agar according to EP/USP/JP (MSA)


Shorthand symbol: MSA
Item number: 40-1166
Format: Petri Dish, 90mm
Colour: Clear and strong pink
Storage conditions: Dry, in closed bag, at 15-22°C
Shelf-life: 3 Months
pH: 7.4 ± 0.2 bei 25°C
Msa
Intented use and fields of application

Test for Staphylococcus aureus. Nutrient medium for microbiological analysis of sterile products. Medium complies with the requirments in the EP/USP/JP (2006).


Typical composition in g/l

in g per 1 litre of Nutrient medium
Pancreatic-digest of Casein peptone5
Pancreatic-digest of Beef Peptone5
Beef Extract1
D(-)-Mannitol10
Sodium chloride75
Phenol Red0.025
Agar15


Microbiological quality control

The microbial performance test carried out in conformity with the requirements of Pharm. Eur. (Microbiological Examination of non-sterile products according Chapter 2.6.13).


Productivity
Incubation conditions: 18 hours at 30–35°C; Inoculum concentration: 10–100 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 6538/WDCM 00032Good growthYellow colonies


Selectivity
Incubation conditions: 18-72 hours at 30–35°C; Inoculum concentration: > 100 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739/WDCM 00012Complete inhibition-


Microbial Contamination
Incubation conditions: 5–7 days, 20–25°C and 5–7 days, 30–35°C

Specification
No microbial contamination


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