Magnesium Chloride Malachite Green Broth according to Rappaport and Vassiliadis (RVM)


Shorthand symbol: RVM
Item number: 60-1157
Format: Tube, 9ml
Colour: Turquoise, transparent
Storage conditions: Dry, in closed bag, at 15-22°C
Shelf-life: 4 Months
pH: 5.2 ± 0.2 bei 25°C
1157 magnesiumchlorid malachitgr%c3%bcn bouillon nach rappaport und vassiliadis rvm xebios
Intented use and fields of application

Medium for the selective isaoltion and cultivation of Salmonella in foodstuffs and other materials.


Typical composition in g/l

in g per 1 Litre of Nutrient medium
Soya and Casein Peptone5
Magnesium Chloride, anhydrous18.73
Sodium Chloride8
Potassium Dihydrogen Phosphate1.6
Malachite Green0.04


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of DIN EN ISO 11133:2014.


Productivity

Incubation Conditions: 24±3 Hours at 41.5±1°C; Inoculum Concentration: 100 CFU

OrganismType StrainSpecificationColony morphology
Salmonella enterica serotype Enteritidis
+ Escherichia coli
+ Pseudomonas aeroginosa
ATCC 13076/WDCM 00030
+ ATCC 8739/WDCM 00012
+ ATCC 27853/WDCM 00025
> 10 colonies from XLD-AgarColonies with a black centre and a lightly transparent reddish zone due to the colour change of the medium(XLD)

Selectivity
Incubation Conditions: 24±3 Hours at 41.5±1°C; Inoculum Concentration: 10,000 - 1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739/WDCM 00012< 100 Colonies in TSA-
Enterococcus faecalisATCC 19433/WDCM 00009< 10 Colonies in TSA-


Microbial Contamination

Incubation Conditions: 5–7 Days, 20–25°C and 5–7 Days, 30–35°C

Specification
No microbial contaminatkion


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