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m-CP-Agar


Shorthand symbol: MCP
Item number: 45-1288
Format: Petri Dish, 70mm
Colour: Violett, transparent
Storage conditions: Dry, in closed bag, at 4-10°C
Shelf-life: 2 Months
pH: 7.4 ± 0.2 bei 25°C
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Intented use and fields of application

MCP Agar (Membrane-Clostridium-Perfringens-Agar) is used for the detection and enumeration of Clostridium perfringens (including spores) in water.


Typical composition in g/l

in g per 1 litre of nutrient medium
Tryptose30
Yeast Extract20
Saccharose5
L-Cysteine Hydrochloride1
Magnesium Sulphate, anhydrous0.048
Indoxyl-ß-Glucoside0.06
Bromocresol Purple0.04
Phenolphthalein-Diphosphate0.1
Ferric (III)Chloride0.09
Cycloserine0.4
Polymyxin B-Sulfat0.025
Agar12.0
*Adjusted as required to meet performance standards


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).

Productivity
Incubation conditions: 2–3 days at 30–35 °C; Inoculum concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Clostridium perfringensATCC 13124 / WDCM 0000750 – 130 %Yellow colonies; Phosphatase Test Positive


Selectivity
Incubation conditions: 21 ± 3 Hours at 44 ± 1 °C; Inoculum concentration: 10,000-1,000,000 CFU, anaerobic incubation

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739 / WDCM 00012Complete inhibition-


Specificity
Incubation conditions: 21 ± 3 Hours at 44 ± 1 °C; Inoculation concentration: 1.000-10.000 CFU, anaerobic incubation

OrganismType StrainSpecificationColony morphology
Clostridium bifermentans NCTC 506 / WDCM 00079-Blue colonies; Phosphatase Test Negative

Microbial Contamination
Incubation conditions: 5–7 days at 20–25 °C and 5–7 days at 30–35 °C

Specification
No microbial contamination


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