m-CP-Agar (MCP)
According to ISO 6461-2:1986
| Shorthand symbol: | MCP |
| Item number: | 40-1288 |
| Format: | Petri Dish, 90mm |
| Colour: | Violet, transparent |
| Storage conditions: | Dry, in closed bag at 4 – 10°C. |
| Shelf-life: | 2 Months |
| pH: | 7.4 ± 0.2 at 25°C |
MCP-Agar (Membrane-Clostridium-Perfringens-Agar) for the detection and enumeration of Clostridium perfringens (including spores) in water.
| in g per 1 Litre of Nutrient medium | |
|---|---|
| Tryptose | 30.0 |
| Yeast Extract | 20.0 |
| Saccharose | 5.0 |
| L-Cysteine Hydrochloride | 1.0 |
| Magnesium Sulphate, anhydrous | 0.048 |
| Indoxyl-ß-Glucoside | 0.06 |
| Bromocresol Purple | 0.04 |
| Phenolphthalein-Diphosphate | 0.1 |
| Ferric (III)Chloride | 0.09 |
| Cycloserine | 0.4 |
| Polymyxine-B Sulphate | 0.025 |
| Agar | 12.0 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).
Productivity
Incubation conditions: 2–3 days at 30–35 °C; Inoculation concentration: 80–120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Clostridium perfringens | ATCC 13124 / WDCM 00007 | 50 – 130 % | Yellow colonies; Phosphatase Test Positive |
Selectivity
Incubation conditions: 21 ± 3 hours at 44 ± 1 °C; Inoculation concentration: 10.000-1.000.000 CFU, anaerobic incubation
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739 / WDCM 00012 | Complete inhibition | - |
Specificity
Incubation conditions: 21 ± 3 Hours at 44 ± 1 °C; Inoculation concentration: 1,000-10,000 CFU, anaerobic incubation
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Clostridium bifermentans | NCTC 506 / WDCM 00079 | - | Blue colonies; Phosphatase Test Negative |
Microbial Contamination
Incubation conditions: 5–7 days at 20–25 °C and 5–7 days at 30–35 °C
| Specification |
|---|
| No microbial contamination |