Membrane-Clostridium-Perfringens-Agar; m-CP-Agar (MCP)

According to ISO 6461-2:1986


Shorthand symbol: MCP
Item number: 40-1288
Format: Petri Dish, 90mm
Colour: Violet, transparent
Storage conditions: Dry, in closed bag, at 4 – 10°C.
Shelf-life: 2 Months
pH: 7.4 ± 0.2 at 25°C
1288 m cp agar mcp xebios diagnostics
Intented use and fields of application

mCP-Agar (Membrane-Clostridium-Perfringens-Agar) for the detection and enumeration of Clostridium perfringens (including spores) in water.


Typical composition in g/l

in g per 1 Litre of Nutrient medium
Tryptose30.0
Yeast Extract20.0
Saccharose5.0
L-Cysteine Hydrochloride1.0
Magnesium Sulphate, anhydrous0.048
Indoxyl-ß-Glucoside0.06
Bromocresol Purple0.04
Phenolphthalein-Diphosphate0.1
Ferric (III)Chloride0.09
Cycloserine0.4
Polymyxine-B Sulphate0.025
Agar12.0


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).


Productivity
Incubation conditions: 2–3 days at 30–35 °C; Inoculation concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Clostridium perfringensATCC 13124 / WDCM 0000750 – 130 %Yellow colonies; Phosphatase Test Positive


Selectivity

Incubation conditions: 21 ± 3 Hours at 44 ± 1 °C; Inoculation concentration: 10,000-1,000,000 CFU, anaerobic incubation

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739 / WDCM 00012Complete inhibition-


Specificity

Incubation conditions: 21 ± 3 Hours at 44 ± 1 °C; Inoculation concentration: 1,000-10,000 CFU, anaerobic incubation

OrganismType StrainSpecificationColony morphology
Clostridium bifermentans NCTC 506 / WDCM 00079-Blue colonies; Phosphatase Test Negative


Microbial Contamination
Incubation conditions: 5–7 days at 20–25 °C and 5–7 days at 30–35 °C

Specification
No microbial contamination


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