Legionella-GVPC-Selektivagar (GVPC)

According to ISO 11731


Shorthand symbol: GVPC
Item number: 40-1602
Format: Petri dish, 90mm
Colour: Black, opaque
Storage conditions: Keep away from light at constant temperature; at 4 – 25°C.
Shelf-life: 3 Months
pH: 6,8 ± 0,2 at 25°C
Gvpc1
Intented use and fields of application

For the detection, isolation and enumeration of Legionella from water samples and clinical material.


Typical composition in g/l

in g per 1l Medium
Yeast Extract10
Activated Charcoal
2
ACES (Buffer/Potassium Hydroxide)10
L-Cysteine0.4
Ferric (III) Pyrophospate0.25
α-Ketoglutarate1
Potassium hydroxide
2.8
Glycine3
Vancomycin0.001
Polymyxin B (in IU)80 000
Cycloheximid0.08
Agar13
*Adjusted as required to meet performance standards


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of ISO 11133.


Productivity
Incubation conditions: 2–5 days and 5–10 days at 36 ± 2 °C; Inoculation concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Legionella pneumophilaATCC 33152 / WDCM 00107>50 %White-grey-blue-violet entire colonies with a smooth edge and a characteristic milky appearance.

Legionella anisa

(5-10 days)

ATCC 35392 / WDCM 00106>50 %White-grey-blue-violet entire colonies with a smooth edge and a characteristic milky appearance.


Selectivity
Incubation conditions: 3 days at 36 ± 2 °C; Inoculation concentration: 10,000-1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739 /
WDCM 00012
Complete or partial inhibition.-
Enterococcus faecalisATCC 19433 /
WDCM 00009
Complete inhibition.-


Microbial Contamination
Incubation conditions: 48 ± 4h  at 30 ± 1°C

Specification
No microbial contamination


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