Legionella-GVPC-Selective-Agar - 70mm-Petri Dish (GVPC)


Shorthand symbol: GVPC
Item number: 45-1502
Format: Petri Dish, 70mm
Colour: Black, opaque
Storage conditions: Dry, in closed bag, at 4-10°C
Shelf-life: 3 Months
pH: 6.9 ± 0.1 bei 25°C
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Intented use and fields of application

For the detection, isolation and enumeration of Legionella from water samples and clinical material.


Typical composition in g/l

per 1 litre of nutrient medium
Activated Charcoal2
Yeast Extract10
ACES (Buffer/Potassium Hydroxide)12.8
L-Cysteine0.4
Ferric (III) Pyrophospate0.25
α-Ketoglutarate1
Glycine3
Vancomycin0.001
Polimyxin B (in IE)79200
Cycloheximide0.08
Agar13


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014.


Productivity
Incubation conditions: 2–5 days and 5–10 days at 36 ± 2 °C; Inoculation concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Legionella pneumophilaATCC 33152 / WDCM 0010750 – 130 %White-green-blue-violet entire colonies with a smooth edge and a characteristic milky appearance.
Legionella anisaATCC 35392 / WDCM 0010650 – 130 %White-green-blue-violet entire colonies with a smooth edge and a characteristic milky appearance.


Selectivity
Incubation conditions: 3 days at 36 ± 2 °C; Inoculation concentration: 10,000-1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739 /
WDCM 00012
Complete or partial inhibition.g-
Pseudomonas
aeruginosa
ATCC 9027 /
WDCM 00026
Complete or partial inhibition.-
Enterococcus faecalisATCC 19433 /
WDCM 00009
Complete inhibition.-


Microbial Contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C

Specification
No microbial contamination


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