Legionella-GVPC-Selective-Agar (GVPC)

According to ISO 11731


Shorthand symbol: GVPC
Item number: 40-1502
Format: Petri Dish, 90mm
Colour: Black, opaque
Storage conditions: Dry, in closed bag, at 4 – 10°C.
Shelf-life: 3 Months
pH: 6.9 ± 0.1 at 25°C
Gvpc1
Intented use and fields of application

For the detection, isolation and enumeration of Legionella from water samples and clinical material.


Typical composition in g/l

in g per 1 Litre of Nutrient medium
Activated Charcoal2.0
Yeast Extract10.0
ACES (Buffer/Potassium Hydroxide)12.8
L-Cysteine0.4
Ferric (III) Pyrophospate0.25
α-Ketoglutarate1.0
Glycine3.0
Vancomycin0.001
Polimyxin B (in IE)79,200
Cycloheximide0.08
Agar13.0


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements ISO 11133:2014.


Productivity
Incubation conditions: 2–5 days and 5–10 days at 36 ± 2 °C; Inoculation concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Legionella pneumophilaATCC 33152 / WDCM 0010750 – 130 %White-grey-blue-violet entire colonies with a smooth edge and a characteristic milky appearance.
Legionella anisaATCC 35392 / WDCM 0010650 – 130 %White-grey-blue-violet entire colonies with a smooth edge and a characteristic milky appearance.


Selectivity
Incubation conditions: 3 days at 36 ± 2 °C; Inoculation concentration: 10,000-1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739 /
WDCM 00012
Complete or partial inhibition.-
Pseudomonas
aeruginosa
ATCC 9027 /
WDCM 00026
Complete or partial inhibition.-
Enterococcus faecalisATCC 19433 /
WDCM 00009
Complete inhibition.-


Microbial Contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C

Specification
No microbial contamination


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