Legionella-BCYE-Agar (mit Cystein) (BCYE+CYS)

According to ISO 11731


Shorthand symbol: BCYE+CYS
Item number: 40-1603
Format: Petri dish, 90mm
Colour: Black, opaque
Storage conditions: Keep away from light at constant temperature; at 4 – 25°C.
Shelf-life: 6 months
pH: 6.8 ± 0.2 at 25°C
Bcye
Intented use and fields of application

For the detection, isolation and enumeration of Legionella from water samples and clinical material.


Typical composition in g/l


in g per 1l Medium
Yeast extract
10
Activated charcoal
2
ACES-Buffer10
L-Cysteine0.4
Iron (III)-pyrophosphate0.25
α-Ketoglutarate1
Potassium hydroxide2.8
Agar13
*Adjusted as required to meet performance standards


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of ISO 11133.


Productivity
Incubation conditions: 2–5 days and 5–10 days at 36 ± 2 °C; Inoculation concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Legionella pneumophilaATCC 33152 / WDCM 00107>50 %White-grey-blue-violet entire colonies with a smooth edge and a characteristic milky appearance.

Microbial Contamination
Incubation conditions: 48 ± 4h  at 30 ± 1°C

Specification
No microbial contamination


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