Legionella-BCYE-Agar / CASO-Agar mit Schafblut
| Shorthand symbol: | BCYE/TSAB |
| Item number: | 42-1555 |
| Format: | Double Petri dish, 90mm |
| Colour: | Black / Blood red |
| Storage conditions: | Dry and closed at 4-10°C |
| Shelf-life: | 4 Weeks |
| pH: | 6.9 ± 0.1 / 7.3 ± 0.2 at 25°C |
Double plate for the isolation of Legionella from non-sterile samples with low incidental flora (BCYEα) as well as for the negative test for the confirmation of Legionella (TSAB).
Legionella-BCYEα-Agar:
| in g per 1l Medium | |
|---|---|
| Activated carbon | 2 |
| Yeast extract | 10 |
| ACES-Buffer | 12.8 |
| L-Cysteine | 0,4 |
| Iron (III)-pyrophosphate | 0.25 |
| α-Ketoglutarate | 1 |
| Agar | 12 |
Caseinpepton-Sojamehlpepton-Agar nach harm. EP/USP/JP mit 5% Schafblut:
| in g per 1l Medium | |
|---|---|
| Peptone from casein | 15 |
| Peptone from Soya | 5 |
| Sodium chloride | 5 |
| Sheep blood, definibriniert | 50 |
| Agar | 12 |
| *Adjusted as required to meet performance standards |
Legionella-BCYEα-Agar:
The microbial performancetest is carried out in conformity with the requirements of DIN EN ISO 11133
Productivity
Incubation conditions: 2 – 5 days at 36 ± 2 °C; Inoculum concentration: 80 – 120 CFU
Organism | Test strain | Specification | Colonymorphology | |
|---|---|---|---|---|
| Legionelle pneumophila | ATCC 33152 / WDCM 00107 | 70 – 130 % | White-gray-blue-violet colored colonies with a smooth border and a frosted glass-like appearance characteristics |
MicrobialContamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
Specification
No microbial contamination
Casein peptone-Soya flour peptone-Agar nach harm. EP/USP/JP with 5% Sheep blood:
The microbial performance test is carried out in conformity with the requirements of DIN EN ISO 11133 .Productivity
Incubation conditions: max. 18 hours at 32.5 ± 2.5°C; Inoculum concentration of the target Organism: 80 – 120 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Staphylococcus aureus | ATCC 6538 / WDCM 00032 | 50 – 130 % | ß-hemolysis around the colonies |
Microbial contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| Specification |
|---|
| No microbial contamination |