According to ISO 11731

Shorthand symbol: BCYE
Item number: 40-1503
Format: Petri Dish, 90mm
Colour: Black, opaque
Storage conditions: Dry, in closed bag at 4 – 10°C.
Shelf-life: 3 Months
pH: 6.9 ± 0.1 at 25°C
Intented use and fields of application

For the detection, isolation and enumeration of Legionella from water samples and clinical material.

Typical composition in g/l

in g per 1 Litre of Nutrient medium
Activated Charcoal2.0
Yeast Extract10.0
ACES (Buffer/Potassium Hydroxide)12.8
Ferric (III)Pyrophosphate0.25
*Adjusted as required to meet performance standards

Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements ISO 11133:2014.

Incubation conditions: 2–5 days and 5–10 days at 36 ± 2 °C; Inoculation concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Legionelle pneumophilaATCC 33152 / WDCM 00107>70%White-grey-blue-violet entire colonies with a smooth edge and a characteristic milky appearance.

Microbial Contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C

No microbial contamination

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