Laurylsulfat-Tryptose-B. (Tryptoph. u. MUG)
| Shorthand symbol: | LTTM |
| Item number: | 60-1153 |
| Format: | Tube, 9ml with Durham tube |
| Colour: | Clear,yellowish colored |
| Storage conditions: | Dry and closed at 15-22°C |
| Shelf-life: | 2 Months |
| pH: | 6.8 ± 0.2 at 25°C |
For detection and germ count determination of coliform bacteria from water, wastewater, food and dairy products.
| in g per 1l Nutrient medium | |
|---|---|
| Tryptose | 20 |
| Lactose | 5 |
| Sodium chloride | 5 |
| Sodium lauryl sulfate | 0.1 |
| Potassium hydrogen phosphate | 2,75 |
| Potassium dihydrogen phosphate | 2.75 |
| MUG(4-Methylumbelliferyl-ß-D-glucuronid) | 0.1 |
| Tryptophan | 1 |
| *Adjusted as required to meet performance standards |
The microbial performance test is carried out in conformity with the requirements of DIN EN ISO 11133.
Productivity
Incubations condition: E. coli: 24 ± 2 to 48 ± 2 hours at 37 ± 1 °C; Coliform bacteria: 24 ± 2 to 48 ± 2 hours at 30 ± 1 °C; Inoculum concentration: 80 – 120 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli (37 ± 1 °C) | ATCC 8739 / WDCM 00012 | Turbidity: + / Gas formation: + | - | |
| Escherichia coli (30 ± 1 °C) | ATCC 8739 / WDCM 00012 | Turbidity: + / Gas formation: + | - | |
| Citrobacter freundii | ATCC 43864 / WDCM 00006 | Turbidity: + / Gas formation: + | - |
Selectivity
Incubation conditions: 24 ± 2 hours at 37 ± 1 °C; Inoculum Concentration: 10.000 – 1.000.000 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Enterococcus faecalis | ATCC 19433 / WDCM 00009 | Complete inhibition | - |
Microbial contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| Specification |
|---|
| No microbial contamination |