Lactose-TTC-Agar with Tergitol-7 (Tergitol-7 TTC Agar) (LTTC)


Shorthand symbol: LTTC
Item number: 40-1278
Format: Petri Dish, 90mm
Colour: Blue-green
Storage conditions: Dry, in closed bag, at 4-10°C
Shelf-life: 3 Months
pH: 7.1 ± 0.2 bei 25°C
Lttc
Intented use and fields of application

Lactose TTC Agar with Tergitol-7 is a selective and differential medium suitable for the isolation, enumeration and identification of Coliform bacteria in food and water samples. Tergitol-7 is added to this medium to inhibit the growth of Gram-Positive bacteria and to suppress the swarming of Proteus species.

Production of acid from the growth of Lactose-fermenting Coliform bacteria results in the colour of the Bromothymol Blue Indicator in the agar to turn yellow and an insoluble red substance formed by the reduction of Triphenyl-tetrazolium Chloride (TTC) by the coliform bacteria.br>


Typical composition in g/l

in g per 1 litre of Nutrient medium
Peptone10
Meat Extract5
Yeast Extract6
Lactose20
Bromthymol Blue0.05
Tergitol-70.1
2,3,5-Tri-phenyltetrazolium Chloride (TTC)0.025
Agar12


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).


Productivity

Incubation conditions: 21 ± 3 Hours at 36 ± 2 °C; Incubation Conditions: 80 – 120 KBE

OrganismType StrainSpecificationColony morphology
Escherichia coliNCTC 13167 / WDCM 0017950 – 130 %Typical, yellow colonies with yellow background
Enterobacter aerogenesATCC 13048 / WDCM 0017550 – 130 %Orange-Yellow colonies
Citrobacter freundiiATCC 43864 / WDCM 0000650 – 130 %Orange-Yellow colonies


Selectivity

Incubation conditions: 21 ± 3 Hours at 36 ± 2 °C; Inoculation concentration: 10,000–1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Enterococcus faecalisATCC 19433 / WDCM 00009Complete inhibition-


Specificity

Incubation conditions: 21 ± 3 Hours at 36 ± 2 °C; Inoculation concentration: 1,000 – 10,000 CFU

OrganismType StrainSpecificationColony morphology
Pseudomonas aeruginosaATCC 27853 / WDCM 00025Good growthRed colonies with blue background

Microbial Contamination
Incubation conditions: 5–7 days at 20–25 °C and 5–7 days at 30–35 °C

Specification
No microbial contamination


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