Lactose-Fuchsin-Sulphite-Agar (Endo-Agar) (ENDO)

Shorthand symbol: ENDO
Item number: 40-1130
Format: Petri Dish, 90mm
Colour: Light pink
Storage conditions: Dry, in closed bag at 4-10°C - avoid exposure to light
Shelf-life: 2 Months
pH: 7.5 ± 0.2 bei 25°C
Dsc 3833 endo web
Intented use and fields of application

Moderately selective differential medium for the isolation and differentiation of Enterobacteriaceae.

The Fuchsin contained in the Lactose-Fuchsin-Sulphite agar is liberated by the reaction with acids and acethaldehydes, both are metabolites of lactose-fermenting bacteria. The liberated Fuchsin results in a red colour with a greenish metallic sheen of the colonies of coliform microorganisms, whereas the Endo agar Dyed similar. In contrast, lactose-negative microorganisms appear in  colourless colonies before the light pink background of the nutrient medium. Simultaneously, the fuchsin sulfite partially inhibits the growth of gram-positive bacteria.

Typical composition in g/l

in g per 1 Litre Nutrient medium
Pancreatic-digest of Peptone (Meat, Gelatine)7.7
Yeast Extract2.3
Dipotassium Hydrogen Phosphate3.0
Potassium Dihydrogen Phosphate0.5
New Fuchsin0.15
Sodium Sulphite1.0
*Adjusted as required to meet performance standards

Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of DIN EN ISO 11133.

Incubation Conditions: 20±4 Hours at 36±2°C; Inoculum Concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 25922/WDCM 0001350–130 %Deep red colonies
Enterobacter aerogenesATCC 13048/WDCM 0017550–130 %Red to reddish, hemispherical, slimy colonies

Incubation Conditions: 20±4 Hours at 37±1°C; Inoculum Concentration: 10,000–1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 25923/WDCM 00034Complete inhibition-

Microbial Contamination
Incubation Conditions: 5–7 Days at 20–25°C and 5–7 Days at 30–35°C

No microbial contamination

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