Lactose-Fuchsin-Sulphite-Agar (Endo-Agar) (ENDO)


Shorthand symbol: ENDO
Item number: 40-1130
Format: Petri Dish, 90mm
Colour: Light pink
Storage conditions: Dry, in closed bag, at 4-10°C - avoid exposure to light
Shelf-life: 2 Months
pH: 7.5 ± 0.2 bei 25°C
Dsc 3833 endo web
Intented use and fields of application

Moderately selective differential medium for the isolation and differentiation of Enterobacteriaceae.

The Fuchsin contained in the Lactose-Fuchsin-Sulphite agar is liberated by the reaction with acids and acethaldehydes, both metabolites of lactose-fermenting bacteria. The liberated Fuchsin results in a red colour with a greenish metallic sheen of the colonies of coliform microorganisms, wherein the Endo agar Dyed similar. In contrast, lactose-negative microorganisms appear colonies colourless before the light pink background of the nutrient medium. Simultaneously, the fuchsin sulfite partially inhibits the growth of gram-positive bacteria.


Typical composition in g/l

in g per 1 Litre Nutrient medium
Pancreatic-digest of Peptone (Meat, Gelatine)7.7
Yeast Extract2.3
Lactose10.0
Dipotassium Hydrogen Phosphate3.0
Potassium Dihydrogen Phosphate0.5
New Fuchsin0.15
Sodium Sulphite1.0
Agar10.0


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of DIN EN ISO 11133.


Productivity
Incubation Conditions: 20±4 Hours at 36±2°C; Inoculum Concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 25922/WDCM 0001350–130 %Deep red colonies
Enterobacter aerogenesATCC 13048/WDCM 0017550–130 %Red to reddish, hemispherical, slimy colonies


Selectivity
Incubation Conditions: 20±4 Hours at 37±1°C; Inoculum Concentration: 10,000–1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 25923/WDCM 00034Complete inhibition-


Microbial Contamination
Incubation Conditions: 5–7 Days, 20–25°C and 5–7 Days, 30–35°C

Specification
No microbial contamination


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