Lactose-Bouillon (Flüssiges Lactose-Medium)
with Durham tubes
| Shorthand symbol: | FLM |
| Item number: | 60-1151 |
| Format: | Tube, 9ml |
| Colour: | Beige, transparent |
| Storage conditions: | Dry and closed at 15-22°C |
| Shelf-life: | 4 Weeks |
| pH: | 6.9 ± 0.2 at 25°C |
The Lactose Broth (Liquid Lactose Medium) with Durham tubes is used to culture and identify coliform bacteria, particularly E. coli, in water, food and raw materials.
| in g per 1l Nutrient medium | |
|---|---|
| Meat extract | 3 |
| Gelatine peptone, pankrease digest | 5 |
| Lactose-Monohydrate | 5 |
| *Adjusted as required to meet performance standards |
The microbial performance test is carried out in conformity with the requirements of DIN EN ISO 11133.
Productivity
Incubation conditions: 44 ± 4 hours at 36 ± 1 °C; Inoculum concentration: ≤ 100 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739 / WDCM 00012 | Significant Turbidity, Gas formation | - | |
| Klebsiella pneumoniae | ATCC 31488 / WDCM 00206 | Significant Turbidity, Gas formation | - | |
| Salmonella enterica serovar Enteritidis | ATCC 13076 / WDCM 00030 | Significant Turbidity | - |
Microbial Contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| Specification |
|---|
| No microbial contamination |