Violet Red Bile Dextrose Agar (VRBD) according to harm. EP/USP/JP (VRBD)

Recommended by the Harmonised European Pharmacopoeia


Shorthand symbol: VRBD
Item number: 40-1042
Format: Petri Dish, 90mm
Colour: Violet, transparent
Storage conditions: Dry, in closed bag, at 4 – 10°C.
Shelf-life: 6 Months
pH: 7.4 ± 0.2 at 25°C
Vrbd
Intented use and fields of application

Violet Red Bile Dextrose Agar (VRBD) medium is for the detection and enumeration bile-tolerant Gram Negative bacteria from foods such as milk and dairy products, egg products and animal carcasses. Moreover VRBD Agar is used for the detection of Enterobacterieceae in meat and meat products. The culture medium complies with the requirements of the method includced in the EP/USP/JP.


Typical composition in g/l

in g per 1 litre Nutrient medium
Yeast Extract3
Pancreatic-digest of Gelatine Peptone7
Bile Salts/td>1.5
Sodium Chloride5
Glucose Monohydrate10
Neutral Red0.03
Crytal Violet0.002
Agar15


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).



Productivity
Incubation conditions: 24±2 hours at 37±1°C; Inoculum concentration: Targer organisms: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Salmonella enterica ssp. AbonyNCTC 6017/WDCM 0002950–130 %Pink-red colonies and no odour and no precipitate
Escherichia coliATCC 8739/WDCM 0001250–130 %Pink-red coloniesand no odour and no precipitate


Selectivity
Incubation conditions: 24±2 hours at 37±1°C; Inoculum concentration: 10,000–1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Enterococcus faecalisATCC 19433/WDCM 00009Complete inhibition-


Microbial Contamination
Incubation conditions: 5–7 days, 20–25°C and 5–7 days, 30–35°C

Specification
No microbial contamination


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