Kligler-Eisen-Agar
| Shorthand symbol: | KIA |
| Item number: | 60-1146 |
| Format: | Tube, Slant agar |
| Colour: | Redish, transparent |
| Storage conditions: | Dry and closed at 15-22°C |
| Shelf-life: | 4 Weeks |
| pH: | 7.8 ± 0.2 at 25°C |
The Kligler iron agar is used for differentiating Gram-negative bacteria, especially Enterobacteriaceae.
| in g pro 1l Nährmedium | |
|---|---|
| Peptone | 23 |
| Yeast extract | 3 |
| Glucose | 1 |
| Lactose | 10 |
| Iron(II)-sulfate | 0.2 |
| Sodium chloride | 5 |
| Sodium thiosulfate | 0.3 |
| Phenol red | 0.05 |
| Agar | 7 |
| *Adjusted as required to meet performance standards |
The microbial performance test is carried out in conformity with the requirements of DIN EN ISO 11133.
Productivity
Incubation conditions:21 ± 3 hours at 37 ± 1 °C; Inoculum concentration: 80 – 120 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739 / WDCM 00012 | Higher layer: yellow Slopy surface: yellow Gas formation: positive | No blackening | |
| Salmonella enterica serovar Enteritidis | ATCC 13076 / WDCM 00030 | Higher layer: yellow slopy surface: red Gas formation: positive | Blackening | |
| Shigella flexneri | ATCC 29903 / WDCM 00125 | Higher layer: yellow Slopy surface: red Gas formation: negative | No blackening | |
| Pseudomonas aeruginosa | ATCC 9027 / WDCM 00026 | Higher layer: red Slopy surface: red Gas formation: negative | No blackening |
Microbial Contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| Specification |
|---|
| No microbial contamination |