King’s B-Agar (KBA)
According to Drinking Water Regulations 2002 and DIN EN 12780
| Shorthand symbol: | KBA |
| Item number: | 60-1287 |
| Format: | Tube, Slant Agar |
| Colour: | Clear to light opaque, light yellow |
| Storage conditions: | Dry, in closed bag, at 15-22°C |
| Shelf-life: | 9 Months |
| pH: | 7.2 ± 0.2 bei 25°C |
For the detection and enumeration of fluorescent Pseudomonas bacteria in water, in particular by Pseudomonas aeruginosa (Drinking Water Regulations 2002 and DIN EN 12780).
| in g per 1 Litre of Nutrient medium | |
|---|---|
| Peptone | 20 |
| Magnesium Sulphate, anhydrous | 0.73 |
| Dipotassium Hydrogen Phosphate | 1.5 |
| Agar | 14.7 |
The Microbiological Performance Test is carried out in accordance with the requirements of DIN EN ISO 11133.
Productivity
Incubation conditions: 44±4 Hours at 36±1°C; Inoculum concentration: 100 CFU; with loosely-fitted cap
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Pseudomonas aeruginosa | ATCC 9027/WDCM 00026 | Good growth | Colour: Greenish-yellow; Fluorescence (at 365nm): Greenish-yellow |
Specificity
Incubation Conditions: 44±4 Hours at 36±1°C; Inoculum Concentration: 1,000–10,000 CFU; with loosely-fitted cap
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739/WDCM 00012 | Good growth | Colour: Colourless; Flouresence (at 365nm): None |
Microbial Contamination
Incubation Conditions: 5–7 Day at 20–25°C and 5–7 Days at 30–35°C
| Specification |
|---|
| No microbial contamination |