Inaktivatorlösung (HIA)

Shorthand symbol: HIA
Item number: 75-1530
Format: 143ml-Wide neck bottle with 90ml Nutrient medium
Colour: light yellow
Storage conditions: Dry, closed, protected from light, at 4-25°C
Shelf-life: 6 Months
pH: 7,0 ± 0,2 at 25°C
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Intented use and fields of application

Inactivating solution is used to inactivate substances in a test sample which could have inhibitory effects on microbiological growth. After an inactivation period of 10 to 15 minutes the sample material can be transferred to other culture media for further investigation.

Typical composition in g/l

in g per 1 Litre of Nutrient medium
Sodium chloride8,5
Sodium thiosulfate pentahydrate 5,0
Potassium dihydrogenphosphate 0,35
Di-potassium hydrogen phosphate anhydrit 0,4
Tween 80 60,0

Microbiological quality control

The product fulfills the customer specific requirements.

Incubation conditions: 15 - 30 Minutes at 20 - 25°C +Transfer (TSA) 40-48 hours at 37±1°C; Inoculation concentration: 10.000 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 6538 / WDCM 00032T1/T0 ≥0,7
Escherichia coliATCC 8739 / WDCM 00012T1/T0 ≥0,7

Microbial Contamination

Incubation conditions: 2 days at 30±1°C

No microbial contamination

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