Inactivation broth (HIA)


Shorthand symbol: HIA
Item number: 75-1530
Format: 143ml-Wide neck bottle with 90ml Nutrient medium
Colour: without colour
Storage conditions: Dry, in closed bag, protected from light, at 15-25°C
Shelf-life: 6 Months
pH: 7,0 ± 0,2 at 25°C
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Intented use and fields of application

Inactivating solution is used to inactivate substances in a test sample which could have inhibitory effects on microbiological growth. After an inactivation period of 10 to 15 minutes the sample material can be transferred to other culture media for further investigation.


Typical composition in g/l

in g per 1 Litre of Nutrient medium
Tryptone1,0
Sodium chloride8,5
Sodium thiosulfate pentahydrate 5,0
Potassium dihydrogenphosphate 0,35
Di-potassium hydrogen phosphate anhydrit 0,4
Tween 80 60,0
L-Histidin2,0
Lecithin6,0


Microbiological quality control

The product fulfills the customer specific requirements.

 
Productivity
Incubation conditions: 15 - 30 Minutes at 20 - 25°C; Inoculation concentration: 10.000 – 100.000 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 6538 / WDCM 00032No change of total germ number-
Escherichia coliATCC 8739 / WDCM 00012No change of total germ number-


Microbial Contamination

Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30 – 35 °C

Specification
No microbial contamination


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