Yeast Extract Agar (YEA)

According to DIN EN ISO 6222:1999

Shorthand symbol: YEA
Item number: 72-1290
Format: 500ml-bottle with 400ml nutrient medium
Colour: Clear, yellowish coloured
Storage conditions: Dry, in closed bag, at 15-22°C
Shelf-life: 3 Months
pH: 7.2 ± 0.2 bei 25°C
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Intented use and fields of application

Yeast Extract Agar Medium is for the detection, isolation and enumeration of bacteria cultivated at 22 ± 1 °C and 37 ± 1 °C in water and waste water samples (BS EN ISO 6222:1999).

Typical composition in g/l

in g per 1 litre nutrient medium
Casein Peptone6.0
Yeast Extract3.0

Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014.


Incubation conditions: 44 ± 4 Hours at 36 ± 1°C; Inoculation concentration of target organisms:80–120 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739 / WDCM 00012≥ 70 %-
Bacillus subtilisATCC 6633 / WDCM 00003≥ 70 %-

Microbial Contamination

Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C

No microbial contamination

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