Galle-Äsculin-Azid-Agar (GÄA)
| Shorthand symbol: | GÄA |
| Item number: | 40-1276 |
| Format: | Petri Dish, 90mm |
| Colour: | Yellowish |
| Storage conditions: | Dry, in closed bag, at 4-10°C |
| Shelf-life: | 4 Months |
| pH: | 7.1 ± 0.2 at 25°C |
Bile Aesculin Azide Agar allows the selective isolation, determination and enumeration Eterococci species in water and other study materials. It is also used for the confirmation of suspect colonies of the Enterococci species selectively isolated on Slanetz & Bartley Agar medium.
| in g per 1 litre of Nutrient medium | |
|---|---|
| Casein peptone (Tryptone) | 17 |
| Peptone | 3 |
| Yeast Extract | 5 |
| Bile salts, dehydrated | 10 |
| Sodium chloride | 5 |
| Aesculin | 1 |
| Ammonium Ferric(III)citrate | 0.5 |
| Sodium azide | 0.15 |
| Agar | 12 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014.
Productivity
Incubation conditions: 44±4 hours at 36±2°C; Inoculum concentration: 80-120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Enterococcus faecalis | ATCC 19433/WDCM 00009 | 50–130 % | Black colonies | |
| Enterococcus faecium | ATCC 6057/WDCM 00177 | 50–130 % | Black colonies |
Selectivity
Incubation conditions: 44±4 hours at 36±2°C; Inoculum concentration: 10.000-1.000.000 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Staphylococcus aureus | ATCC 6538/WDCM 00032 | Complete inhibition | - | |
| Escherichia coli | ATCC 8739/WDCM 00012 | Complete inhibition | - |
Microbial Contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35 °C
| Specification |
|---|