Bile Aesculin Azide Agar (GÄA)


Shorthand symbol: GÄA
Item number: 40-1276
Format: Petri Dish, 90mm
Colour: Yellowish
Storage conditions: Dry, in closed bag, at 4-10°C
Shelf-life: 4 Months
pH: 7.1 ± 0.2 bei 25°C
Dsc 3777 g%c3%84a web
Intented use and fields of application

Bile Aesculin Azide Agar allows the selective isolation, determination and enumeration Eterococci species in water and other study materials. It is also used for the confirmation of suspect colonies of the Enterococci species selectively isolated on Slanetz & Bartley Agar medium.


Typical composition in g/l

in g per 1 litre of Nutrient medium
Casein peptone (Tryptone)17
Peptone3
Yeast Extract5
bile salts, dehydrated10
Sodium chloride5
Aesculin1
Ammonium Ferric(III)citrate0.5
Sodium azide0.15
Agar12


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014.


Productivity
Incubation conditions: 44±4 hours at 36±2°C; Inoculum concentration: 80-120 CFU

OrganismType StrainSpecificationColony morphology
Enterococcus faecalisATCC 19433/WDCM 0000950–130 %Black colonies
Enterococcus faeciumATCC 6057/WDCM 0017750–130 %Black colonies


Selectivity
Incubation conditions: 44±4 hours at 36±2°C; Inoculum concentration: 10,000-1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 6538/WDCM 00032Complete inhibition-
Escherichia coliATCC 8739/WDCM 00012Complete inhibition-


Microbial Contamination
Incubation conditions: 5–7 days, 20–25°C and 5–7 days, 30–35 °C

Specification


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