Enterococci Selective Agar according to Slanetz and Bartley - 70mm-Petri Dish (ESB)

According to DIN EN ISO 7899-2:2000 and § 64 LFGB


Shorthand symbol: ESB
Item number: 45-1132
Format: Petri Dish, 70mm
Colour: Yellowish to light red
Storage conditions: Dry, in closed bag at 4-10°C
Shelf-life: 3 Months
pH: 7.2 ± 0.2 at 25°C
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Intented use and fields of application

Slanetz and Bartley Selective Agar for the isolation and identification of Enterococci in water (BS EN ISO 7899-2:2000).

The Sodium Azide contained in the Slanetz and Bartley Selective Agar inhibits non-target microbiological flora ensuring a high selectivity for Enterococci. Tri-phenyl-tetrazolium chloride is added to the medium so that Enterococci colonies are visualised as red, maroon or pink coloured colonies.

The composition of this medium meets the requirements of the Microbiology of Drinking Water (MoDW) 2012 Part 5 and BS EN ISO 7899-2:2000.


Typical composition in g/l

in g per 1 litre of Nutrient medium
Tryptose20
Yeast Extract5
Glucose2
Di-Potassium Hydrogen Phosphate4
Sodium Azide0.4
2,3,5-Tri-Phenyl-Tetrazolium Chloride (TTC)0.1
Agar12
*Adjusted as required to meet performance standards


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014.


Productivity
incubation conditions: 44 ± 4 hours at 36 ± 2 °C; Inoculum concentration: 80 – 120 CFU

OrganismType StrainSpecificationColony morphology
Enterococcus faecalisATCC 19433 / WDCM 0000950 – 130 %Red, maroon, pink colonies
Enterococcus faeciumATCC 6057 / WDCM 0017750 – 130 %Red, maroon, pink colonies


Selectivity
Incubation conditions: 44 ± 4 hours at 36 ± 2 °C; Inoculum concentration: 10.000 – 1.000.000 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 6538 / WDCM 00032Complete inhibition-
Escherichia coliATCC 8739 / WDCM 00012Complete inhibition-


Microbial Contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C

Specification
No microbial contamination


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