Enterokokken-Selektiv-Agar nach Slanetz-Bartley (ESB)
According to ISO 7899-2:2000
| Shorthand symbol: | ESB |
| Item number: | 40-1132 |
| Format: | Petri Dish, 90mm |
| Colour: | Yellowish to light red |
| Storage conditions: | Dry, in closed bag at 4 – 10°C. |
| Shelf-life: | 4 Months |
| pH: | 7.2 ± 0.2 at 25°C |
Slanetz and Bartley Selective Agar for the isolation and identification of Enterococci in water. The Sodium Azide contained in the Slanetz and Bartley Selective Agar inhibits non-target microbiological flora ensuring a high selectivity for Enterococci. Tri-phenyl-tetrazolium chloride is added to the medium so that Enterococci colonies are visualised as red, maroon or pink coloured colonies.
| in g per 1 Litre of Nutrient medium | |
|---|---|
| Tryptose | 20.0 |
| Yeast Extract | 5.0 |
| Glucose | 2.0 |
| Di-Potassium Hydrogen Phosphate | 4.0 |
| Sodium Azide | 0.4 |
| 2,3,5-Tri-Phenyl-Tetrazolium Chloride (TTC) | 0.10 |
| Agar | 10.0 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance Test is carried out in accordance with the requirements ISO 11133:2014.
Productivity
incubation conditions: 44 ± 4 hours at 36 ± 2 °C; Inoculum concentration: 80 – 120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Enterococcus faecalis | ATCC 19433 / WDCM 00009 | 50 – 130 % | Red, maroon, pink colonies | |
| Enterococcus faecium | ATCC 6057 / WDCM 00177 | 50 – 130 % | Red, maroon, pink colonies |
Selectivity
Incubation conditions: 44 ± 4 Hours at 36 ± 2 °C; Inoculum concentration: 10.000 – 1.000.000 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Staphylococcus aureus | ATCC 6538 / WDCM 00032 | Complete inhibition | - | |
| Escherichia coli | ATCC 8739 / WDCM 00012 | Complete inhibition | - |
Microbial Contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| Specification |
|---|
| No microbial contamination |