DG18-Agar mit Chloramphenicol (DG18)
According to ISO 21527-2
| Shorthand symbol: | DG18 |
| Item number: | 50-1038 |
| Format: | Contact plate |
| Colour: | Beige, transparent |
| Storage conditions: | Dry, in closed bag, at 15-22°C |
| Shelf-life: | 3 Months |
| pH: | 5.6 ± 0.2 bei 25°C |
DG18 Agar (Dichloran Glycerol Agar) with Chloramphenicol is for the isolation and cultivation of xerophilic fungi according to ISO 21527-2. The addition of chloramphenicol is added to inhibit bacterial growth, particularly that of Gram-negative bacteria.
| in g per 1 Litre Nutrient medium | |
|---|---|
| Casein Peptone | 5 |
| D-Glucose | 10 |
| Potassium Dihydrogen Phosphate | 1 |
| Dichloran | 0.002 |
| Magnesium Sulphate, anhydrous | 0.4 |
| Chloramphenicol | 0.1 |
| Agar | 14 |
The Microbiological Performance Test is carried out in accordance with the requirements of DIN EN ISO 11133:2014.
Productivity
Incubation Conditions: 5 Days at 25±1°C; Inoculum Concentration of Target Organisms: 80–120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Saccharomyces cerevisiae | ATCC 9763/WDCM 00058 | 50–130% | Whitish, dry colonies | |
| Wallemia sebi | ATCC 42694/WDCM 00182 | 50–130% | Brown colonies |
Selectivity
Incubation Conditions: 5 Days at 25±1°C; Inoculum Concentration of Target Organisms: 10,000 – 1,000,000 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739/WDCM 00012 | Complete inhibition | - | |
| Bacillus subtilis | ATCC 6633/WDCM 00003 | Complete inhibition | - |
Microbial Contamination
Incubation Conditions: 5–7 Days, 20–25°C and 5–7 Days, 30–35°C
| Specification |
|---|
| No microbial contamination |