DG18-Agar with Chloramphenicol (DG18)

According to ISO 21527-2


Shorthand symbol: DG18
Item number: 40-1038
Format: Petri Dish, 90mm
Colour: Beige, transparent
Storage conditions: Dry, in closed bag, at 4 – 10°C.
Shelf-life: 3 Months
pH: 5.6 ± 0.2 at 25°C
1038 dg18 agar mit chloramphenicol dg18 xebios 2
Intented use and fields of application

Dichloran-Glycerol-Agar mit Chloramphenicol is used for isolation and enumeration of xerophilic moulds according to ISO 21527-2. The addition of Chloramphenicol inhibits the growth of bacteria, especially Gram Negative bacteria.


Typical composition in g/l

in g per 1 Litre Nutrient medium
Casein Peptone5
D-Glucose10
Potassium Dihydrogen Phosphate1
Dichloran0.002
Magnesium Sulphate, anhydrous0.4
Chloramphenicol0.1
Agar14


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of DIN EN ISO 11133:2014.


Productivity

Incubation Conditions: 5 Days at 25±1°C; Inoculum Concentration of Target Organisms: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Saccharomyces cerevisiaeATCC 9763/WDCM 0005850–130%Whitish, dry colonies
Wallemia sebiATCC 42694/WDCM 0018250–130%Brown colonies


Selectivity

Incubation Conditions: 5 Days at 25±1°C; Inoculum Concentration of Target Organisms: 10,000 – 1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739/WDCM 00012Complete inhibition-
Bacillus subtilisATCC 6633/WDCM 00003Complete inhibition-


Microbial Contamination

Incubation Conditions: 5–7 Days, 20–25°C and 5–7 Days, 30–35°C

Specification
No microbial contamination


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