DEV Nutrient Agar (DEV)

According to DIN 38411

Shorthand symbol: DEV
Item number: 40-1128
Format: Petri Dish, 90mm
Colour: Light yellowish
Storage conditions: Dry, in closed bag at 15 – 22°C.
Shelf-life: 6 Months
pH: 7.3 ± 0.2 at 25°C
Intented use and fields of application

DEV Nutrient Agar is used for the detection, isolation and enumeration of bacteria from food and water. The medium corresponds to the requirements DIN 38411.

Typical composition in g/l

in g per 1 Litre of Nutrient medium
Peptone from flesh, tryptic-digested10.0
Beef Extract10.0
Sodium Chloride5.0
*Adjusted as required to meet performance standards

Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements ISO 11133:2014.


Incubation conditions: 44 ± 4 Hours at 36 ± 1°C; Inoculum concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739 / WDCM 00012≥ 70 %Yellowish/Cream colonies
Bacillus subtilisATCC 6633 / WDCM 00003≥ 70 %Yellowish/Cream colonie

Microbial Contamination

Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C

No microbial contamination

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