Columbia-Blutagar (CBA)

Shorthand symbol: CBA
Item number: 40-1118
Format: Petri Dish, 90mm
Colour: blood-red
Storage conditions: Dry, in closed bag at 4 – 10°C.
Shelf-life: 2 Months
pH: 7.3 ± 0.2 at 25°C
Intented use and fields of application

Used for the cultivation of fastidious microorganisms and for determining their haemolysis

Typical composition in g/l

in g per 1l Medium
Sodium Chloride5.0
Sheep's blood, defibrinated50.0
*Adjusted as required to meet performance standards

Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and Pharm. Eur. (Microbiological Examination of non-sterile products in accordance with Chapter 2.6.13).

Incubation conditions: 2-3 days at 30-35°C; Inoculum concentration: Target organisms: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 25923/WDCM 0003450-130%-
Bacillus cereusATCC 11778/WDCM 0000150-130%-
Escherichia coliATCC 8739/WDCM 0001250-130%-
Bacillus subtilisATCC 6633/WDCM 0000350-130%-
Listeria MonocytogenesATCC 13932/WDCM 0002150-130%-

Microbial Contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C

No microbial contamination

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