Columbia-Blood-Agar (with 5% sheep's blood) (CBA)


Shorthand symbol: CBA
Item number: 40-1118
Format: Petri Dish, 90mm
Colour: blood-red
Storage conditions: Dry, in closed bag, at 4 – 10°C.
Shelf-life: 2 Months
pH: 7.3 ± 0.2 at 25°C
Cba
Intented use and fields of application

Used for the cultivation of fastidious microorganisms and for determining their haemolysis


Typical composition in g/l

in g per 1 Litre of Nutrient medium
Peptone23.0
Starch1.0
Sodium Chloride5.0
Sheep's blood, defibrinated50.0
Agar11.0


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and Pharm. Eur. (Microbiological Examination of non-sterile products in accordance with Chapter 2.6.13).


Productivity
Incubation conditions: 2-3 days at 30-35°C; Inoculum concentration: Target organisms: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 25923/WDCM 0003450-130%-
Bacillus cereusATCC 11778/WDCM 0000150-130%-
Escherichia coliATCC 8739/WDCM 0001250-130%-
Bacillus subtilisATCC 6633/WDCM 0000350-130%-
Listeria MonocytogenesATCC 13932/WDCM 0002150-130%-


Microbial Contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C

Specification
No microbial contamination


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