Clostridien-Differential-Bouillon
| Shorthand symbol: | DRCM |
| Item number: | 60-1116 |
| Format: | Tube, 9ml |
| Colour: | Red brown, transparent |
| Storage conditions: | Dry, closed at 15-22°C |
| Shelf-life: | 4 Weeks |
| pH: | 7.1 ± 0.2 at 25°C |
The Clostridia Differential Broth is used to determine the colony count of clostridia according to the MPN technique in foods and other materials.
| in g per 1l Nutrient medium | |
|---|---|
| Meat peptone | 10 |
| Meat extract | 10 |
| Yeast extract | 1.5 |
| Soluble starch | 1 |
| Glucose | 1 |
| Sodium acetate | 5 |
| L-Cysteine hydrochloride | 0.5 |
| sodium sulfite | 0.4 |
| Iron (III)-citrate | 0.7 |
| Resazurin | 0.002 |
Die mikrobielle Leistungsprüfung erfolgt in Konformität mit den Anforderungen der DIN EN ISO 11133.
Productivity
Incubation conditions: 44 ± 4 hour at 36 ± 1 °C under anaerobic Atmospher; Inoculum concentration: ≤ 100 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Clostridium perfringens | ATCC 13124 / WDCM 00007 | Significant cloudy | Black color |
Specificity
Incubation conditions: 44 ± 4 hours at 36 ± 1 °C under anaerobic Atmospher; Inoculum concentrationn: 1.000 – 10.000 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739 / WDCM 00012 | Bright cloudy | No black color |
Microbial Contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| Specification |
|---|
| No microbial contamination |