Casein Peptone-Soya Peptone Broth according to the harmonised EP/USP/JP (TSB)


Shorthand symbol: TSB
Item number: 60-1263
Format: Tube, 9ml
Colour: Clear, yellowish coloured
Storage conditions: Dry, in closed bag, at 15-22°C
Shelf-life: 3 Months
pH: 7.3± 0.2 bei 25°C
Tsb
Intented use and fields of application

Universal Nutrient Medium for the cultivation of bacteria, yeasts and fungi. The compoenents Casein Peptone and Soya Peptone provide the optimal growth conditions for many sensitive microorganisms. This culture medium complies with the recommendations of the harmonised method according to EP/USP/JP (2006).


Typical composition in g/l

in g per 1 litre of Nutrient medium
Caseine Peptone, pancreatic-digested17
Soya Peptone, papaine-digested3
Sodium Chloride5
Dipotassium Hydrogenphosphate2.5
Glucose Monohydrate2.5


Microbiological quality control

The microbial performance test is carried out in accordance with the requirements of DIN EN ISO 11133.


Productivity
Incubation Conditions: max. 18 Hours at 32.5±2.5°C; Inoculum Concentration of Target Organisms: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739/WDCM 00012Very cloudy/turbid-
Staphylococcus aureusATCC 25923/WDCM 00034Very cloudy/turbid-
Pseudomonas aeruginosaATCC 10145/WDCM 00024Very cloudy/turbid-
Bacillus subtilisATCC 6633/WDCM 00003Very cloudy/turbid-
Enterococcus faecalisATCC 29212/WDCM 00087Very cloudy/turbid-
Clostridium perfringensATCC 13124/WDCM 00007Very cloudy/turbid-
Listeria monocytogenesATCC 13932/WDCM 00021Very cloudy/turbid-
Bacillus cereusATCC 11778/WDCM 00001Very cloudy/turbid-


Microbial Contamination
Incubation conditions: 5–7 Days at 20–25°C and 5–7 Days at 30–35°C

Specification
No microbial contamination


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