Tryptic Soy Agar acc. to EP/USP/JP (with 5% sheep's blood) (TSAB)


Shorthand symbol: TSAB
Item number: 40-1505
Format: Petri Dish, 90mm
Colour: blood-red
Storage conditions: Dry, in closed bag at 4 – 10°C.
Shelf-life: 2 Months
pH: 7.3 ± 0.2 at 25°C
Tsab
Intented use and fields of application

For the isolation and cultivation of various fastidious microorgansism, especially pathogenic microorganisms, and for determining their haemolysis.


Typical composition in g/l

in g per 1 Litre of Nutrient medium
Casein Peptone15.0
Soya Peptone5.0
Sodium Chloride5.0
Sheep's blood, defibrinated50.0
Agar15.0
*Adjusted as required to meet performance standards


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).

Productivity
Incubation conditions: 2–3 days at 30–35 °C; Inoculum concentration: 10–100 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 6538/WDCM 0003250–200 %-
Pseudomonas aeruginosaATCC 9027/WDCM 0002650–200 %-
Bacillus subtilisATCC 6633/WDCM 0000350–200 %-
Candida albicansATCC 10231/WDCM 0005450–200 %-
Aspergillus brasiliensisATCC 16404/WDCM 0005350–200 %-
Candida albicans
(3–5 days, 20–25°C)
ATCC 10231/WDCM 0005450–200 %-
Aspergillus brasiliensis
(3–5 days, 20–25°C)
ATCC 16404/WDCM 0005350–200 %-

Microbial contamination
Incubation conditions: 5–7 days at 20–25 °C and 5–7 days at 30–35°C

Specification
No microbial contamination


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