Tryptic Soy Agar acc. to EP/USP/JP (with 5% sheep's blood) (TSAB)


Shorthand symbol: TSAB
Item number: 40-1505
Format: Petri Dish, 90mm
Colour: blood-red
Storage conditions: Dry, in closed bag, at 4 – 10°C.
Shelf-life: 2 Months
pH: 7.3 ± 0.2 at 25°C
Tsab
Intented use and fields of application

For the isolation and cultivation of various fastidious microorgansism, especially pathogenic microorganisms, and for determining their haemolysis.


Typical composition in g/l

in g per 1 Litre of Nutrient medium
Casein Peptone15.0
Soya Peptone5.0
Sodium Chloride5.0
Sheep's blood, defibrinated50.0
Agar15.0


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).

Productivity
Incubation conditions: 2–3 days at 30–35 °C; Inoculation concentration: 10–100 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 6538/WDCM 0003250–200 %-
Pseudomonas aeruginosaATCC 9027/WDCM 0002650–200 %-
Bacillus subtilisATCC 6633/WDCM 0000350–200 %-
Candida albicansATCC 10231/WDCM 0005450–200 %-
Aspergillus brasiliensisATCC 16404/WDCM 0005350–200 %-
Candida albicans
(3–5 days, 20–25°C)
ATCC 10231/WDCM 0005450–200 %-
Aspergillus brasiliensis
(3–5 days, 20–25°C)
ATCC 16404/WDCM 0005350–200 %-

Microbial contamination
Incubation conditions: 5–7 days, 20–25 °C and 5–7 days, 30–35°C

Specification
No microbial contamination


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