Tryptic Soy Agar acc. to EP/USP/JP (TSA)
Recommended by the Harmonised European Pharmacopoeia
|Format:||Petri Dish, 90mm|
|Storage conditions:||Dry, in closed bag, at 15 – 22°C.|
|pH:||7,3 ± 0,2 at 25°C|
Tryptone Soya Agar is a very nutrient-rich universal growth for the microbiological examination of non-sterile products. A general purpose agar medium, containing two peptones, which will support the growth of a wide variety of organisms. It is suitable for the cultivation both of aerobic and anaerobic bacteria, the latter being grown either in deep cultures or by incubation under anaerobic conditions as well as yeasts and moulds.
The high numbers of microorganisms detectable using Tryptone Soya Agar is due to the addition of peptones gained from enzumatic hydrolysis of casein protein and soya proteins. It therfore includes - besides others - the detection of Listeria spp, Pasteurella spp, Vibrio spp, Haemophilus vaginalis or Candida spp.
Tryptone Soya Agar contains no carbohydrates so it can be used in the investigation of haemolytic reactions. The Pharmacopeia European (PhEur) recommends TSA for the enumeration of Total Viable Count in products under examination for microbial load.
|in g per 1 litre of medium|
|Pancreatic-digest of Casein Peptone||15.0|
|Enzyme-digest of Soya Bean Peptone||5.0|
The Microbiological Performance Test is carried out in accordance with the requirements of ISO 11133:2014 and/or PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).
Incubation conditions: 2–3 days at 30–35 °C; Inoculation concentration: 80–120 CFU
|Organism||Type Strain||Specification||Colony morphology|
|Pseudomonas aeruginosa||ATCC 9027 / WDCM 00026||>70%||Medium-sized, yellowish colonies|
|Escherichia coli||ATCC 8739 / WDCM 00012||>70%||Yellow colonies|
|Clostridium perfringens||ATCC 13124 / WDCM 00007 (anaerobe Bebrütung)||>70 %||White colonies|
|Enterococcus faecalis||ATCC 29212 / WDCM 00087||>70 %||White colonies|
Incubation conditions: 5–7 days at 20–25 °C and 5–7 days at 30–35 °C
|No microbial contamination|