Tryptone Soya Agar (TSA-LTH)

Shorthand symbol: TSA-LTH
Item number: 50-1531
Format: Contact plate
Colour: Yellow
Storage conditions: Dry, in closed bag, at 15 – 22°C.
Shelf-life: 6 Months
pH: 7.3 ± 0.2 bei 25°C
1531 abklatschplatte caseinpepton sojamehlpepton agar mit enthemmer tsa lth xebios
Intented use and fields of application

Tryptone Soya Agar is a highly nutritious universal medium for the testing non-sterile products. It is suitable as an inhibitor-free culture medium for the isolation and cultivation of various fastidious bacteria, yeasts and moulds (aerobic and anaerobic).

The variety of detectable microorganisms with Tryptone Soya Agar is due to he casein protein components in the nutrient medium being enzymatically hydrolysed into peptones. This makes it possible to isolate and detect bacteria including: Listeria spp, Pasteurella spp, Vibrio spp, Haemophilus vaginalis or Candida albicans.

Tryptone Soya Agar does not contain any carbohydrate, so it can be used for the study of hemolytic reactions. The European Pharmacopeia recommends Tryptone Soya Agar for total bacteria enumeration in products under examination by this pour or spread plate technique.

Die Addition of lecithin, Tween 80 and histidine neutralises the inhibitory effect on growth caused by aldehydes contained in many detergents and disinfectants, phenols, Hexachlorophene, chlorhexidine, formaldehyde and quaternary ammonium compounds used in preservatives and biocides.

Typical composition in g/l

in g per 1 litre of Nutrient Medium
Pancreatic-digest of Casein Peptone15.0
Enzyme-digest of Soya Bean Pepton5.0
Sodium Chloride 5.0
Lecithin 0.7
Tween 80 5.0
L-Histidine 0.5

Microbiological quality control

the Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13.

Incubation conditions: 2–3 days at 30–35°C; Inoculum concentration: 10–100 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 6538 / WDCM 0003250–200 %Medium, slightly yellowish colonies
Pseudomonas aeruginosaATCC 9027 / WDCM 0002650–200 %Medium, slightly yellowish colonies
Bacillus subtilisATCC 6633 / WDCM 0000350–200 %Large, flat, dry, irregularly shaped colonies
Candida albicansATCC 10231 / WDCM 0005450–200 %Small, white, dry colonies
Aspergillus brasiliensisATCC 16404 / WDCM 0005350–200 %Dark brown to black conidia on light mycelium
Candida albicans
(3–5 days at 20–25°C)
ATCC 10231 / WDCM 0005450–200 %Small, white, dry colonies
Aspergillus brasiliensis
(3–5 days at 20–25 °C)
ATCC 16404 / WDCM 0005350 – 200 %Dark brown to black conidia on light mycelium

Microbial contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35 °C

No growth present

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