Brilliant Green-Phenol Red-Agar acc. to Edel and Kampelmacher (BPLS-Agar mod.) (BPLS)


Shorthand symbol: BPLS
Item number: 40-1110
Format: Petri Dish, 90mm
Colour: Reddish-brown
Storage conditions: Dry, in closed bag, at 4 – 10°C.
Shelf-life: 3 Months
pH: 7.0 ± 0.2 at 25°C
Bpls
Intented use and fields of application

Modified Brilliant Green Phenol Red Agar according to Edel and Kampelmacher is a selective medium for the isolation of Salmonella from foods. The medium has been modified by increasing the Brilliant Green concentration so that it shows good growth of Gram Positive Bacteria whereas most Gram Negative bacteria are strongly inhibited.


Typical composition in g/l

in g per 1 litre of Nutrient medium
Peptone10.0
Beef Extract5.0
Yeast Extract3.0
Lactose10.0
Saccharose10.0
Disodium dihydrogen phosphate1.0
Sodium dihydrogen phosphate0.6
Brillant Green0.005
Phenol Red0.09
Agar10.0


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of ISO 11133:2014.


Productivity
Incubation conditions: 24±3 hoursa at 37±1°C; Inoculum concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Salmonella enterica ssp. abonyNCTC 6017/WDCM 0002950–130 %Pink colonies


Selectivity
Incubation conditions: 24±3 hours at 37±1°C; Inoculum concentration: 10.000–1.000.000 CFU

OrganismType StrainSpecificationColony morphology
Enterococcus faecalisATCC 19433/WDCM 00009Complete inhibition-
Escherichia coliATCC 6538/WDCM 00012Growth and partial inhibitionYellow colonies


Mikrobial Contamination
Incubation conditions: 5–7 days, 20–25°C and 5–7 days, 30–35°C

Specification
No microbial contamination


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