Brilliantgrün-Phenolrot-Agar n. Edel-Kampelmacher (BPLS)
| Shorthand symbol: | BPLS |
| Item number: | 40-1110 |
| Format: | Petri Dish, 90mm |
| Colour: | Reddish-brown |
| Storage conditions: | Dry, in closed bag at 4 – 10°C. |
| Shelf-life: | 3 Months |
| pH: | 7.0 ± 0.2 at 25°C |
Modified Brilliant Green Phenol Red Agar according to Edel and Kampelmacher is a selective medium for the isolation of Salmonella from foods. The medium has been modified by increasing the Brilliant Green concentration so that it shows good growth of Gram Positive Bacteria, whereas most Gram Negative bacteria are strongly inhibited.
| in g per 1 litre of Nutrient medium | |
|---|---|
| Peptone | 10.0 |
| Beef Extract | 5.0 |
| Yeast Extract | 3.0 |
| Lactose | 10.0 |
| Saccharose | 10.0 |
| Disodium dihydrogen phosphate | 1.0 |
| Sodium dihydrogen phosphate | 0.6 |
| Brillant Green | 0.005 |
| Phenol Red | 0.09 |
| Agar | 10.0 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance Test is carried out in accordance with the requirements of ISO 11133:2014.
Productivity
Incubation conditions: 24±3 hoursa at 37±1°C; Inoculum concentration: 80–120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Salmonella enterica ssp. abony | NCTC 6017/WDCM 00029 | 50–130 % | Pink colonies |
Selectivity
Incubation conditions: 24±3 hours at 37±1°C; Inoculum concentration: 10.000–1.000.000 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Enterococcus faecalis | ATCC 19433/WDCM 00009 | Complete inhibition | - | |
| Escherichia coli | ATCC 6538/WDCM 00012 | Growth and partial inhibition | Yellow colonies |
Mikrobial Contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C
| Specification |
|---|
| No microbial contamination |