BPLS-Agar mod. / XLD-Agar nach harm. EP/USP/JP
| Shorthand symbol: | BPLS/XLD |
| Item number: | 42-1560 |
| Format: | Double Petri dish, 90mm |
| Colour: | Reddish-Brown |
| Storage conditions: | Dry and closed at 4-10°C |
| Shelf-life: | 2 Months |
| pH: | 7.0 ± 0.2 / 7.4 ± 0.2 at 25°C |
For the isolation of salmonella from food.
Brilliant green-Phenol-Agar according to Edel and Kampelmacher:
| in g per 1l Medium | |
|---|---|
| Peptone | 10 |
| Meat extract | 5 |
| Yeast extract | 3 |
| Lactose | 10 |
| Sucrose | 10 |
| Disodium hydrogen phosphate | 1 |
Sodium dihydrogen phosphate | 0.6 |
| Brillant green | 0.005 |
| Phenol red | 0.09 |
| Agar | 10 |
Xylose-Lysine-Deoxycholate Agar nach EP/USP/JP:
| in g per 1l Medium | |
|---|---|
| Yeast extract | 3.0 |
| L-Lysine | 5.0 |
| Xylose | 3.5 |
| Lactose-Monohydrate | 7.5 |
| Sucrose | 7.5 |
| Sodium deoxycholate | 2.5 |
| Sodium thiosulphate | 6.8 |
| Ammonium Iron (III)-citrate | 0.8 |
| Phenol red | 0.08 |
| sodium chloride | 5.0 |
| Agar | 13.5 |
| *Adjusted as required to meet performance standards |
The microbial performancetest is carried out in conformity with the requirements of DIN EN ISO 11133.
Brilliant green-Phenol-Agar according to Edel and Kampelmacher:
Productivity
Incubation conditions: 24 ± 3 hours at 37 ± 1 °C; Inoculum concentration: 80 – 120 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Salmonella enterica ssp. abony | NCTC 6017 / WDCM 00029 | 50 – 130 % | Red colonies, Red medium |
Selectivity
Incubation conditions: 44 ± 3 hours at 37 ± 1 °C; Inoculum concentration>: 104 – 106 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Enterococcus faecalis | ATCC 19433 / WDCM 00009 | Complete inhibition | - | |
| Escherichia coli | ATCC 8739 / WDCM 00012 | Growth or Partial inhibition | - |
Microbial Contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| Specification |
|---|
| No microbial contamination |
Xylose-Lysine-Deoxycholate Agar according to EP/USP/JP:
The microbial performance test is carried out in conformity with the requirementsof DIN EN ISO 11133 and the Pharm. Eur. (Microbiological testing of non-sterileproducts according to Chapter 2.6.13).
Productivity
Incubation conditions: 24 ± 3 hours at 37 ± 1 °C; Inoculum concentration: 10 – 100 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Salmonella enterica serovar Abony | NCTC 6017 / WDCM 00029 | Good growth | Colonies with a black center and a slightly transparent reddish zone due to the color change of the medium | |
| Salmonella enterica serovar Enteritidis | ATCC 13076 / WDCM 00030 | 50 – 130 % | Colonies with a black center and a slightly transparent reddish zone due to the color change of the medium |
Selectivity
Incubation conditions: 24 ± 3 hours at 37 ± 1 °C; Inoculum concentration: 10.000 – 1.000.000 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739 / WDCM 00012 | Complete inhibition | - | |
| Enterococcus faecalis | ATCC 19433 / WDCM 00009 | Complete inhibition | - |
Microbial Contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| Specification |
|---|
| No microbial contamination |