Brilliantgrün-Galle-Lactose-Bouillon
according to § 64 LFGB, formerly §35 LMBG
| Shorthand symbol: | BGL |
| Item number: | 60-1109 |
| Format: | Vial, 9ml |
| Colour: | clear, green bottle |
| Storage conditions: | Dry, closed at 15-22 ° C |
| Shelf-life: | 3 Months |
| pH: | 7.2 ± 0.2 at 25°C |
Brilliant green bile lactose broth is used for the selective enrichment of Escherichia coli and other coliforms. The bile contained in the nutrient medium and the brilliant green suppress the growth of gram-positive (Flora) completely. The detection of the coliforms takes place via the gas formation as a result of the fermentation of the lactose contained in the nutrient medium.
| in g per 1l Medium | |
|---|---|
| Casein pepton (in g) | 10 |
| OX bile (in g) | 20 |
| Lactose (in g) | 10 |
| Brilliant green (in g) | 0.0133 |
| *Adjusted as required to meet performance standards |
The microbial performance test is carried out in conformity with the requirements of DIN EN ISO 11133.
Productivity
Incubation conditions: 24 ± 2 to 48 ± 2 hours at 30 ± 1 ° C; Inoculum Concentration: 80 - 120 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739 / WDCM 00012 | Turbidity: + | - | |
| Citrobacter freundii | ATCC 43864 / WDCM 00006 | Turbidity: + | - |
Selectivity
Incubations conditions: 24 ± 2 bis 48 ± 2h at 30 ± 1 °C; Inoculum Concentration: 10.000 - 1.000.000 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Enterococcus faecalis | ATCC 19433 / WDCM 00009 | Partial inhibition | - |
Microbial contamination
Incubations condition: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| Specification |
|---|
| No microbiological contamination |